Immune Sensing of Aspergillus fumigatus Proteins, Glycolipids, and Polysaccharides and the Impact on Th Immunity and Vaccination
Immune Sensing of Aspergillus fumigatus Proteins, Glycolipids, and Polysaccharides and the Impact on Th Immunity and Vaccination
复制标题
DOI：
10.4049/jimmunol.0900961
复制
发表时间：
2009-08-15
影响因子：
4.4
通讯作者：
Romani, Luigina
中科院分区：
文献类型：
--
作者：
Bozza, Silvia;Clavaud, Cecile;Romani, Luigina
烟曲霉在体内生命周期中通过细胞壁结构的动态变化和分泌来激活、抑制或颠覆宿主免疫反应的能力，意味着对不同真菌成分的差异性免疫识别。在这项研究中，我们比较评估了分泌型和膜锚定型蛋白质、糖脂以及多糖在移植小鼠中诱导疫苗依赖性保护以及由人特异性CD4(+) T细胞克隆产生Th细胞因子的能力。结果表明，不同的真菌成分在小鼠和人类中具有激活Th细胞反应的不同能力，分泌型蛋白质诱导Th2细胞激活，膜蛋白诱导Th1/Treg，糖脂诱导Th17，而多糖主要诱导IL - 10产生。有趣的是，并排比较显示，至少三种真菌成分（一种蛋白酶和两种糖基磷脂酰肌醇锚定蛋白）在从小鼠到人类中都保留了其免疫优势的Th1/Treg激活潜能。这表明，可以利用特定的免疫活性烟曲霉抗原选择性地开发人类针对该真菌的T细胞库的广度和特异性。《免疫学杂志》，2009年，183卷：2407 - 2414页
The ability of the fungus Aspergillus fumigatus to activate, suppress, or subvert host immune response during life cycle in vivo through dynamic changing of cell wall structure and secretion implicates discriminative immune sensing of distinct fungal components. In this study, we have comparatively assessed secreted- and membrane-anchored proteins, glycolipids, and polysaccharides for the ability to induce vaccine-dependent protection in transplanted mice and Th cytokine production by human-specific CD4(+) T cell clones. The results show that the different fungal components are endowed with the distinct capacity to activate Th cell responses in mice and humans, with secreted proteins inducing Th2 cell activation, membrane proteins Th1/Treg, glycolipids Th17, and polysaccharides mostly IL-10 production. Of interest, the side-by-side comparison revealed that at least three fungal components (a protease and two glycosylphosphatidylinositol-anchored proteins) retained their immunodominant Th1/Treg activating potential from mice to humans. This suggests that the broadness and specificity of human T cell repertoire against the fungus could be selectively exploited with defined immunoactive Aspergillus Ags. The Journal of Immunology, 2009, 183: 2407-2414.