Histometric Analysis of the Effect of Enamel Matrix Derivative on the Healing of Periodontal Defects in Rats With Diabetes
Histometric Analysis of the Effect of Enamel Matrix Derivative on the Healing of Periodontal Defects in Rats With Diabetes
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DOI：
10.1902/jop.2012.120354
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发表时间：
2013-09-01
影响因子：
4.3
通讯作者：
Sallum, Enilson A.
中科院分区：
文献类型：
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作者：
Correa, Monica Grazieli;Gomes Campos, Mirella L.;Sallum, Enilson A.
背景资料：糖尿病（DM）涉及代谢变化，可对牙周组织产生负面影响，导致牙周修复受损。有一个缺乏信息的影响下，DM的再生方法的结果。牙釉质基质衍生物（EMD）已被用于牙周再生程序，导致临床参数的改善。因此，本组织形态计量学研究的目的是评估愈合后的牙周缺损与EMD治疗DM的影响下。方法：20只Wistar大鼠随机分为两组：组1（G1）：DM诱导与单次腹腔注射链脲佐菌素（STZ）（n = 10）;组2（G2）：大鼠没有暴露于STZ（n = 10）。DM诱导后7天，在第一下颌磨牙的颊侧创建双侧开窗缺损。手术后，每只动物的缺损被随机分配到两个亚组：未处理（对照）和用EMD处理。21天后对动物实施安乐死，并通过组织学方法评估缺损填充（DF）、新形成骨密度（BD）和新牙骨质形成（NCF）的百分比。用抗酒石酸酸性磷酸酶测定破骨细胞的数量。还分析了体重和血糖。使用Mann-Whitney U检验进行组间比较，使用Wilcoxon检验进行开始和结束时间（体重和血糖）之间以及治疗之间（NCF和破骨细胞数量）的比较。单因素方差分析用于评估DF和BD。结果：G1组糖尿病患者的DF和BD均低于G2组，差异有统计学意义（α = 5%）。EMD在两组中均增加了DF，仅在G2中增强了BD和NCF。在G1期，EMD处理的部位TRAP阳性破骨细胞数量明显增加。结论：DM对BD有明显的损害作用。EMD在糖尿病或正常情况下可提供更大的DF;然而，它可能不会显著增加DM动物的NCF。
Background: Diabetes mellitus (DM) involves metabolic changes that can negatively influence periodontal tissues, resulting in impaired periodontal repair. There is a lack of information about the outcomes of regenerative approaches under the influence of DM. Enamel matrix derivatives (EMDs) have been used in periodontal regenerative procedures, resulting in improvement of clinical parameters. Thus, the aim of this histomorphometric study is to evaluate the healing of periodontal defects after treatment with EMD under the influence of DM.Methods: Twenty Wistar rats were randomly assigned to two groups: group 1 (G1): DM was induced with a single intraperitoneal injection of streptozotocin (STZ) (n = 10); group 2 (G2): rats were not exposed to STZ (n = 10). Seven days after DM induction, bilateral fenestration defects were created at the buccal aspect of the first mandibular molar. After the surgeries, the defects of each animal were randomly assigned to two subgroups: non-treated (control) and treated with EMD. The animals were euthanized 21 days later, and the percentage of defect fill (DF), newly formed bone density (BD), and new cementum formation (NCF) were histometrically assessed. The number of osteoclasts was determined by tartrate-resistant acid phosphatase. Weight and blood glucose were also analyzed. Mann-Whitney U test was used for comparison among groups and Wilcoxon test for comparison between the start and end times (weight and blood glucose) and between treatments (NCF and number of osteoclasts). One-way analysis of variance was used to assess DF and BD. Tukey test was used when the analysis of variance test detected significant differences (alpha = 5%).Results: G1 (DM) showed less DF and BD compared with G2. EMD provided an increased DF in both groups and enhanced BD and NCF only in G2. The number of TRAP-positive osteoclasts was significantly higher in EMD-treated sites of G1.Conclusions: DM may produce a significant detrimental effect on BD. EMD may provide greater DF under diabetic or normal conditions; however, it may not significantly increase NCF in animals with DM.