Methods for Imaging Labeled Neurons Together with Neuropil Features in Drosophila
Methods for Imaging Labeled Neurons Together with Neuropil Features in Drosophila
复制标题
果蝇标记神经元与神经纤维层特征的成像方法
DOI：
10.1177/002215540004801114
复制
发表时间：
2000
影响因子：
3.2
通讯作者：
Darren W. Williams
中科院分区：
文献类型：
--
作者：
N. Tyrer;D. Shepherd;Darren W. Williams
我们描述了果蝇中央神经节连续半薄切片的染色方案，通过反染色来显示神经节结构，可以可视化特定神经元的基因表达。绿色荧光蛋白（GFP）表达于一个选定的增强子诱捕线的感觉神经元子集中，通过常规的免疫组织化学与过氧化物酶连接的抗体可见，并通过还原银染色显示神经结构。这使得在组织学切片上可以看到与共聚焦显微镜下相同的gfp标记细胞，但特别的优势是，在单个细胞和神经元过程的水平上也可以显示神经细胞结构。这不仅允许细胞内标记神经元之间的物理关系通过参考neuropil中的特定特征来确定，而且还允许将功能暂时归因于neuropil的特定区域。这些方法对于绘制中枢神经系统结构背景下特定神经元的形态信息具有特殊的实用性，无论是在成年果蝇还是在发育过程中。
We describe staining protocols for serial semithin sections of Drosophila central ganglia that allow visualization of gene expression in particular neurons with counter-staining to display the ganglion architecture. Green fluorescent protein (GFP), expressed in a subset of sensory neurons from a selected enhancer trap line, is visualized by conventional immunohistochemistry with a peroxidase-linked antibody, and neural architecture is revealed by reduced silver staining. This makes visible in histological sections the same GFP-labeled cells seen with confocal microscopy, but with the especial advantage that neuropil structures are also revealed at the level of individual cells and neuron processes. Not only does this allow the physical relationships among intracellularly labeled neurons to be determined by reference to specific features in the neuropil but it also enables a function to be ascribed provisionally to particular regions of neuropil. These methods have particular utility for mapping morphological information on specific neurons in the context of central nervous system architecture, both in adult Drosophila and during development.
荧光黄抗血清：制备、表征以及用于充满染料的视网膜神经元的免疫细胞化学定位的用途。
DOI：
10.1177/39.11.1918929
发表时间：
1991
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Brandon,C;Criswell,MH
通讯作者：
Criswell,MH