Indole-inducible proteins in bacteria suggest membrane and oxidant toxicity
Indole-inducible proteins in bacteria suggest membrane and oxidant toxicity
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DOI：
10.1007/s002030050012
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发表时间：
2000-01-01
影响因子：
2.8
通讯作者：
Yukawa, H
中科院分区：
文献类型：
--
作者：
Garbe, TR;Kobayashi, M;Yukawa, H
吲哚的氧化毒性通过诱导大肠杆菌K12中的烷基过氧化氢还原酶亚基C（AhpC）和通过在大肠杆菌K12的变体中的AhpC的组成性过量产生来证明。coli JM 109对吲哚的抗性增强。在黄色短杆菌的吲哚适应变体中，通过吲哚诱导的一种新的36-kDa蛋白质的过量产生也表明了氧化毒性，该蛋白质的N-末端序列与涉及超氧化物和单线态氧抗性的蛋白质相似。提出了吲哚在膜脂质中的破坏，这导致膜紊乱并使氧化还原循环类异戊二烯醌和分子氧直接相互作用成为可能，导致产生超氧化物。E.大肠杆菌中可能是吲哚诱导的原生质球蛋白y（Spy）的过量产生。
Oxidant toxicity of indole was demonstrated by the induction of alkylhydroperoxide reductase subunit C (AhpC) in Escherichia coli K12 and by the constitutive overproduction of AhpC in a variant of E. coli JM109 with enhanced resistance to indole. Oxidant toxicity was also indicated in an indole-adapted variant of Brevibacterium flavum by the indole-inducible overproduction of a novel 36-kDa protein with N-terminal sequence similarity to proteins involved in superoxide and singlet oxygen resistance, It is proposed that indole disserved in membrane lipids, which caused membrane derangement and enabled direct interaction of redox-cycling isoprenoid quinones and dioxygen, resulting in the generation of superoxide. A direct indication of membrane derangement in E. coli may be the indole-inducible overproduction of spheroplast protein y (Spy).