A novel brain receptor is expressed in a distinct population of olfactory sensory neurons
A novel brain receptor is expressed in a distinct population of olfactory sensory neurons
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DOI：
10.1046/j.1460-9568.2000.00286.x
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发表时间：
2000-11-01
影响因子：
3.4
通讯作者：
Strotmann, J
中科院分区：
文献类型：
--
作者：
Conzelmann, S;Levai, O;Strotmann, J
三个新的G蛋白偶联受体基因相关的先前描述的RA1c基因已被分离出小鼠基因组。这些基因的表达已经在大脑的不同区域以及鼻子的嗅上皮中被检测到。发育研究揭示了表达的差异发病：在胚胎阶段17的大脑中，在阶段E12的嗅觉系统。为了确定嗅上皮中哪种细胞类型表达这种独特的受体类型，采用了转基因方法，该方法允许组织学标志物与受体一起共表达，从而使适当的细胞群可视化。结果发现，受体表达细胞位于非常接近的上皮基底膜，然而，细胞延伸的树突状突起的上皮表面和它们的轴突投射到主嗅球，在那里他们聚集到两个或三个肾小球的背侧和后部区域的球。因此，这些数据提供的证据表明，这种独特类型的受体在成熟的嗅觉神经元中表达，并表明它可能参与检测特殊的气味分子。
Three novel G-protein-coupled receptor genes related to the previously described RA1c gene have been isolated from the mouse genome. Expression of these genes has been detected in distinct areas of the brain and also in the olfactory epithelium of the nose. Developmental studies revealed a differential onset of expression: in the brain at embryonic stage 17, in the olfactory system at stage E12. In order to determine which cell type in the olfactory epithelium expresses this unique receptor type, a transgenic approach was employed which allowed a coexpression of histological markers together with the receptor and thus visualization of the appropriate cell population. It was found that the receptor-expressing cells were located very close to the basal membrane of the epithelium; however, the cells extended a dendritic process to the epithelial surface and their axons projected into the main olfactory bulb where they converged onto two or three glomeruli in the dorsal and posterior region of the bulb. Thus, these data provide evidence that this unique type of receptor is expressed in mature olfactory neurons and suggests that it may be involved in the detection of special odour molecules.