Patterns of genetic structure among Hawaiian corals of the genus Pocillopora yield clusters of individuals that are compatible with morphology
Patterns of genetic structure among Hawaiian corals of the genus Pocillopora yield clusters of individuals that are compatible with morphology
复制标题
DOI：
10.1016/j.crvi.2007.12.003
复制
发表时间：
2008-03-01
影响因子：
2
通讯作者：
Tillier, Simon
中科院分区：
文献类型：
--
作者：
Flot, Jean-Francois;Magalon, Helene;Tillier, Simon
六个可变序列标记的开发和分析，以找出种属Pocillopora的夏威夷珊瑚的边界：推定的线粒体控制区;最近发现的，高变线粒体开放阅读框架;内部转录间隔区2（ITS 2），位于核核糖体DNA;三个核内含子的钙调蛋白，延伸因子-1 α和ATP合成酶β亚基。使用前两个标记，我们确定了五个不同的线粒体谱系，这些谱系是兼容的形态。核标记的情况更为复杂，因为在一些个体中观察到两种以上的单倍型。为了检测个体的集群，构建了单倍型网络，其中在共同出现的单倍型之间绘制了额外的连接，以描绘重组的潜在领域：发现很少有核单倍型集群与个体集群相对应，但检测到的那些（主要在ITS 2数据集中）也与形态学兼容。
Six variable sequence markers are developed and analyzed to find out species boundaries in Hawaiian corals of the genus Pocillopora: the putative mitochondrial control region; a recently discovered, hypervariable mitochondrial open reading frame; the internal transcribed spacer 2 (ITS2), located in the nuclear ribosomal DNA; three nuclear introns of calmodulin, elongation factor- 1 alpha and the ATP synthase beta subunit. Using the first two markers, we identify five distinct mitochondrial lineages and these lineages are compatible with morphology. The situation is more complex with nuclear markers since more than two haplotypes are observed in some individuals. To detect clusters of individuals, haplotype networks are constructed with additional connections drawn between co-occurring haplotypes to delineate potential fields for recombination: few clusters of nuclear haplotypes are found to correspond to clusters of individuals, but those that are detected (mostly in the ITS2 dataset) are also compatible with morphology.