F1-dependent translation of mitochondrially encoded Atp6p and Atp8p subunits of yeast ATP synthase
F1-dependent translation of mitochondrially encoded Atp6p and Atp8p subunits of yeast ATP synthase
复制标题
DOI：
10.1073/pnas.0910351106
复制
发表时间：
2009-11-03
影响因子：
11.1
通讯作者：
Tzagoloff, Alexander
中科院分区：
文献类型：
--
作者：
Rak, Malgorzata;Tzagoloff, Alexander
酵母线粒体的ATP合成酶由17种不同的亚基多肽组成。我们已经筛选了一组ATP合成酶突变体，以发现其线粒体编码的唯一亚单位Atp6p、Atp8p和Atp9p的表达受损。我们的结果表明，Atp6p和Atp8p的翻译是由F-1 ATPase(或其组装中间体)激活的。缺乏F-1的α或β亚基，或促进F-1组装的Atp11p和Atp12p伴侣的突变体，具有正常水平的双顺反子ATP8/ATP6 mRNAs，但不能合成Atp6p和Atp8p。当线粒体DNA中正常核基因Arg8(M)被替换为ATP6或ATP8时，F-1突变体也不能表达Arg8(M)。ATP22是一种Atp6p特异的翻译因子，它能够在没有F-1的情况下恢复Atp6p的合成，并在较小程度上恢复Atp8p的合成，这也支持了F-1的翻译激活。这些结果建立了一种机制，通过该机制，ATP6和ATP8的表达受到F-1的翻译调控，以实现两组分开的ATP合成酶基因的平衡输出。
The ATP synthase of yeast mitochondria is composed of 17 different subunit polypeptides. We have screened a panel of ATP synthase mutants for impaired expression of Atp6p, Atp8p, and Atp9p, the only mitochondrially encoded subunits of ATP synthase. Our results show that translation of Atp6p and Atp8p is activated by F-1 ATPase (or assembly intermediates thereof). Mutants lacking the alpha or beta subunits of F-1, or the Atp11p and Atp12p chaperones that promote F-1 assembly, have normal levels of the bicistronic ATP8/ATP6 mRNAs but fail to synthesize Atp6p and Atp8p. F-1 mutants are also unable to express ARG8(m) when this normally nuclear gene is substituted for ATP6 or ATP8 in mitochondrial DNA. Translational activation by F-1 is also supported by the ability of ATP22, an Atp6p-specific translation factor, to restore Atp6p and to a lesser degree Atp8p synthesis in the absence of F-1. These results establish a mechanism by which expression of ATP6 and ATP8 is translationally regulated by F-1 to achieve a balanced output of two compartmentally separated sets of ATP synthase genes.