Muscle-specific microtubule-associated protein 4 is expressed early in myogenesis and is not sufficient to induce microtubule reorganization.
Muscle-specific microtubule-associated protein 4 is expressed early in myogenesis and is not sufficient to induce microtubule reorganization.
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肌肉特异性微管相关蛋白 4 在肌发生早期表达，不足以诱导微管重组。
DOI：
10.1002/cm.10105
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
Olmsted,JoannaB
中科院分区：
文献类型：
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作者：
Casey,LiamM;Lyon,HiramD;Olmsted,JoannaB
使用同种异构体特异性抗体分析了c2c12细胞肌肉形成过程中微管相关蛋白4 （mMAP4）肌肉特异性变体的表达。MMAP4定位于微管（MTs），在单核梭形细胞形成的早期形态发生事件之前表达。MMAP4蛋白与titin同时出现，与高尔基重组时间一致，但早于肌凝蛋白的表达。EGFP - mMAP4在非肌肉细胞和增殖的c2c12细胞中的错误表达不会引起MT组织或稳定性的显著变化，也不会引起高尔基组织的显著变化。全长mMAP4或缺乏MT结合结构域的截断形式的表达不会破坏肌管形成或肌原纤维形成。虽然之前的反义研究表明，mMAP4是正常肌管形成所必需的[Mangan和Olmsted， 1996: Development122:771-781]，但这些数据表明，mMAP4不足以诱导MTs或高尔基体重组为肌肉细胞的典型模式。因此，就MT组织特性而言，这种组织特异性变异不同于相关的神经元MAPs、MAP2和tau，后者可诱导MT组织中的神经样变化。细胞Motil。中国生物医学工程学报，2003。©2003 Wiley‐Liss, Inc。
The expression of a muscle‐specific variant of microtubule‐associated protein 4 (mMAP4) has been analyzed during myogenesis of C2C12cells using an isoform‐specific antibody. MMAP4 localizes to microtubules (MTs) and is expressed prior to a very early morphogenetic event, the formation of mononucleate spindle‐shaped cells. MMAP4 protein appears at about the same time as titin and coincident with Golgi reorganization, but antedates myosin expression. Misexpression of EGFP‐mMAP4 in non‐muscle and proliferating C2C12cells does not induce dramatic changes in MT organization or stability, nor in Golgi organization. Expression of full‐length mMAP4 or of a truncated form lacking the MT‐binding domain does not disrupt myotube formation or myofibrillogenesis. While previous antisense studies indicated that mMAP4 is necessary for normal myotube formation [Mangan and Olmsted, 1996:Development122:771–781], these data indicate mMAP4 is not sufficient to induce the reorganization of MTs or the Golgi into patterns typical of muscle cells. Thus, with respect to MT organizing properties, this tissue‐specific variant differs from related neuronal MAPs, MAP2, and tau, which induce neural‐like changes in MT organization. Cell Motil. Cytoskeleton 54:317–336, 2003. © 2003 Wiley‐Liss, Inc.