Monoclonal antibody against NRP-1 b1b2.
Monoclonal antibody against NRP-1 b1b2.
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DOI：
10.1089/hyb.2011.0012
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发表时间：
2011-08
期刊：
影响因子：
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通讯作者：
Xiang Li;Fanghong Luo;Shengyu Wang;Erru Ni;Xiaoyong Tang;Haiyan Lv;Xiaoli Chen;Lu Chen;Jianghua Yan
中科院分区：
文献类型：
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作者：
Xiang Li;Fanghong Luo;Shengyu Wang;Erru Ni;Xiaoyong Tang;Haiyan Lv;Xiaoli Chen;Lu Chen;Jianghua Yan
Neuropilin-1 是神经毡蛋白家族蛋白的成员，包含一个大的胞外结构域（a1a2、b1b2 和 c）、一个跨膜结构域和一个短的胞质尾。 NRP-1 在血管生成中发挥着关键作用，并通过将 VEGF(165) 与 b1b2 结构域结合来刺激内皮细胞分裂和迁移。在本研究中，我们报道了通过杂交瘤方法建立了特异性针对NRP-1 b1b2的单克隆抗体（A6-26-11-26克隆）。蛋白质印迹分析表明，我们的 NRP-1 b1b2 MAb 可以结合源自肿瘤细胞的 NRP-1 b1b2 和 NRP-1。这种针对 NRP-1 b1b2 的单克隆抗体将有助于进一步开发癌症靶点策略。
Neuropilin-1 is a member of the neuropilins family protein, which contains a large extracellular domain (a1a2, b1b2 and c), a single transmembrane domain, and a short cytoplasmic tail. NRP-1 plays a critical role in angiogenesis and stimulates endothelial cell division and migration by binding VEGF(165) with b1b2 domain. In the present study, we report the establishment of a monoclonal antibody (A6-26-11-26 clone) specific for NRP-1 b1b2 through hybridoma method. Western blot analysis indicates that our NRP-1 b1b2 MAb can combine both NRP-1 b1b2 and NRP-1 originated from tumor cells. This monoclonal antibody against NRP-1 b1b2 will be useful in the further development of cancer target strategy.