Distribution of amniotic stem cells in human term amnion membrane
Distribution of amniotic stem cells in human term amnion membrane
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羊膜干细胞在人足月羊膜中的分布
DOI：
10.1093/jmicro/dfab035
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发表时间：
2021
期刊：
影响因子：
1.8
通讯作者：
Yoshida Toshiko
中科院分区：
文献类型：
--
作者：
Koike Nobuyuki;Sugimoto Jun;Okabe Motonori;Arai Kenichi;Nogami Makiko;Okudera Hiroshi;Yoshida Toshiko
羊膜与流产相关的研究已经在妇产科领域进行。然而，干细胞在羊膜内的分布以及每种类型干细胞性质的差异仍然没有很好地了解。我们在本研究中解决了这一知识空白，我们对羊膜进行了形态学分类，并澄清了干细胞的分布，以识别功能不同的羊膜源性干细胞。羊膜可分为与脐带相连的部位（A区）、附着于胎盘的部位（B区）和位于胎盘对面的部位（C区）。我们发现，强烈表达干细胞标志物的人羊膜上皮干细胞（HAECs）在A区丰富。HAEC不仅表达干细胞特异性表面标志物TRA-1-60、Tra-1-81、SSEA 4、SSEA 3，而且OCT-3/4阳性，并具有碱性磷酸酶活性。人羊膜间充质干细胞表达KLF-A、OCTA、Oct 3/4、c-MYC和转录因子Sox 2。尤其是在A区和B区表达了CD 73，并且药物排泄转运蛋白BCRP的表达高于其他部位。这些数据表明，不同类型的干细胞可能存在于不同的区域。了解干细胞在每个区域和功能中的特征的关系将允许有效收获合适的HAE和HAM干细胞作为再生医学的工具。
Amnion membrane studies related to miscarriage have been conducted in the field of obstetrics and gynecology. However, the distribution of stem cells within the amnion and the differences in the properties of each type of stem cells are still not well understood. We address this gap in knowledge in the present study where we morphologically classified the amnion membrane, and we clarified the distribution of stem cells here to identify functionally different amniotic membrane–derived stem cells. The amnion can be divided into a site that is continuous with the umbilical cord (region A), a site that adheres to the placenta (region B), and a site that is located opposite the placenta (region C). We found that human amnion epithelial stem cells (HAECs) that strongly express stem cell markers were abundant in area A. HAEC not only expressesed stem cell-specific surface markers TRA-1-60, Tra-1-81, SSEA4, SSEA3, but was also OCT-3/4 positive and had alkaline phosphatase activity. Human amniotic mesenchymal stem cells expressed KLF-A, OCTA, Oct3/4, c-MYC and Sox2 which is transcription factor. Especially, in regions A and B they have expressed CD73, and the higher expression of BCRP which is drug excretion transporter protein than the other parts. These data suggest that different types of stem cells may have existed in different area. The understanding the relation with characteristics of the stem cells in each area and function would allow for the efficient harvest of suitable HAE and HAM stem cells as using tool for regenerative medicine.