CONTROL OF THE MICROBIAL-FLORA OF THE VAGINA BY H2O2-GENERATING LACTOBACILLI
CONTROL OF THE MICROBIAL-FLORA OF THE VAGINA BY H2O2-GENERATING LACTOBACILLI
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DOI：
10.1093/infdis/164.1.94
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发表时间：
1991-07-01
影响因子：
6.4
通讯作者：
WALTERSDORPH, AM
中科院分区：
文献类型：
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作者：
KLEBANOFF, SJ;HILLIER, SL;WALTERSDORPH, AM
产生h2o2的乳酸菌（LB+）存在于大多数正常女性的阴道中，但在大多数细菌性阴道病（BV）女性中没有。高浓度LB+对阴道加德纳菌（BV女性阴道内的优势菌）有毒性；当LB+降低到单独无效的水平时，加入髓过氧化物酶和氯化物重新产生毒性。过氧化氢酶对乳酸菌的毒性有抑制作用，而过氧化氢阴性乳酸菌的毒性未见明显变化，提示过氧化氢为毒性分子。LB+可以被H2O2取代，氯可以被碘化物、溴化物或硫氰酸盐取代。最佳抑菌pH为5.0 ~ 6.0。当与髓过氧化物酶和氯化物联合使用时，LB+也具有自身抑制作用。低浓度的LB+通过形成H2O2对拟杆菌（Bacteroides bivius）产生毒性。21名女性中有17名的阴道中发现了足够量的过氧化物酶。这些发现表明LB+可能有助于控制阴道菌群，特别是在过氧化物酶和卤化物存在的情况下。
H2O2-generating lactobcailli (LB+) are present in the vagina of most normal women but are absent from most women with bacterial vaginosis (BV). LB+ at high concentration was toxic to Gardnerella vaginalis (the predominant organism in the vagina of women with BV); when the LB+ was lowered to a level where it was ineffective alone, the addition of myeloperoxidase and chloride reinstituted toxicity. Toxicity was inhibited by catalase and was not seen when H2O2-negative lactobacilli were used, implicating H2O2 as the toxic molecule. LB+ could be replaced by H2O2 and chloride by iodide, bromide, or thiocyanate. The optimum pH for inhibition of G. vaginalis was 5.0-6.0. LB+ also was autoinhibitory when combined with myeloperoxidase and chloride. LB+ alone at low concentrations was toxic to Bacteroides bivius through the formation of H2O2. Adequate amounts of peroxidase were found in the vagina of 17 of 21 women. These findings suggest that LB+ may contribute to the control of the vaginal flora, particularly in the presence of peroxidase and a halide.