A generic approach to evaluate how B-cell epitopes are surface-exposed on protein structures.
A generic approach to evaluate how B-cell epitopes are surface-exposed on protein structures.
复制标题
DOI：
10.1016/j.molimm.2010.10.011
复制
发表时间：
2011-01
影响因子：
3.6
通讯作者：
Tessier D
中科院分区：
文献类型：
--
作者：
Lollier V;Denery-Papini S;Larré C;Tessier D
预测抗体表位的方法可以通过在对抗原的免疫应答期间影响抗体的表位结合来帮助促进诊断工具、疫苗或免疫疗法的开发。通常认为抗体对抗原的可及性与蛋白质的可及表面之间存在直接关系。基于这一假设，预测系统通常包括从蛋白质的一级序列或从其三维结构计算的溶剂可及性值作为预测标准。然而，目前的预测系统似乎弱有效的基准测试。我们感兴趣的是评估已被实验鉴定为表位元件的氨基酸如何在表面暴露水平上与抗原分子的其余部分不同，因此我们评估了表位的平均可及性。这里使用的方法利用从许多鉴定技术推导出的已发表的表位，包括序列扫描和晶体学后的结构可视化，并且它涉及从毒素到过敏原的许多类型的抗原。我们的研究结果表明，表位残基不分布在任何特定的相对表面可及性和蛋白质指数值，在某些情况下，表位覆盖整个抗原序列。这些结果导致的结论是，已知的表位相对于用于识别它们的实验条件的分类，应该在尝试以通用的方式识别表位区域之前引入。
Methods that predict antibody epitopes could help to promote the development of diagnostic tools, vaccines or immunotherapies by affecting the epitope binding of antibodies during an immunological response to antigens. It is generally assumed that there is a direct relationship between antibody accessibility to antigens and accessible surface of proteins. Based on this assumption, prediction systems often includes solvent accessibility values calculated from the primary sequence of proteins or from their three dimensional structures as a predictive criterion. However, the current prediction systems seem weakly efficient in view of benchmark tests. We were interested in evaluating how amino acids that have been experimentally identified as epitopic elements could differ from the rest of the antigenic molecule at the level of surface exposure, hence we assessed the average accessibility of epitopes. The approach used here utilises published epitopes deduced from numerous identification techniques, including sequence scanning and structure visualisation after crystallography, and it involves many types of antigens from toxins to allergens. Our results show that epitopic residues are not distributed among any specific Relative Surface Accessibility and Protrusion Index values and that, in some cases, epitopes cover the entire antigenic sequence. These results led to the conclusion that the classification of known epitopes with respect to the experimental conditions used to identify them should be introduced before attempting to characterise epitopic areas in a generic way.
登录
查看更多内容
影响因子：
14.2
作者：
Albrecht, Melanie;Kuehne, Yvonne;Vieths, Stefan
通讯作者：
Vieths, Stefan
影响因子：
2.9
作者：
Pollastri, G;Baldi, P;Casadio, R
通讯作者：
Casadio, R
影响因子：
5.6
作者：
LEE, B;RICHARDS, FM
通讯作者：
RICHARDS, FM
影响因子：
14.2
作者：
Cocco, RR;Järvinen, KM;Beyer, K
通讯作者：
Beyer, K
影响因子：
14.2
作者：
Lopez-Torrejon, Gema;Diaz-Perales, Araceli;Pacios, Luis F.
通讯作者：
Pacios, Luis F.