Effect of cyclic stretch on alpha-subunit mRNA expression of Na+-K+-ATPase in aortic smooth muscle cells.
Effect of cyclic stretch on alpha-subunit mRNA expression of Na+-K+-ATPase in aortic smooth muscle cells.
复制标题
循环拉伸对主动脉平滑肌细胞中 Na -K -ATP 酶 α 亚基 mRNA 表达的影响。
DOI：
10.1152/ajpcell.2001.280.6.c1555
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Songu-Mize,E
中科院分区：
文献类型：
--
作者：
Sevieux,N;Alam,J;Songu-Mize,E
We previously demonstrated that protein expression of both α1- and α2-catalytic subunits of the Na+-K+-ATPase is elevated after a 2- to 4-day chronic cyclic stretch regimen in cultured aortic smooth muscle cells (ASMC). In the present study, we investigated whether cyclic stretch affects mRNA expression of the α-isoforms of the Na+-K+-ATPase. Using a stretch apparatus, rat ASMC were cyclically stretched 10 or 20% of their length for 1, 3, or 6 h. α-Isoform mRNA levels were measured using Northern analysis. A 3-h 10% stretch had no significant affect on mRNA expression for either isoform, but a 20% stretch increased mRNA of both isoforms approximately twofold. Whereas a 6-h 20% stretch increased α1mRNA by 3.3-fold, α2was not affected any further. Actinomycin D blocked the stretch-induced stimulation of mRNA expression of both α-subunits. In conclusion, cyclic stretch stimulates the mRNA expression of both α1- and α2-subunits of Na+-K+-ATPase. The sensitivity of the two genes to the degree and duration of stretch is different. The stretch-induced increase of mRNA may be a result of increased transcription.
