Effect of inoculation rate of selected strains of lactic acid bacteria on fermentation and in vitro digestibility of grass-legume forage.
Effect of inoculation rate of selected strains of lactic acid bacteria on fermentation and in vitro digestibility of grass-legume forage.
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乳酸菌筛选菌株接种率对草豆类饲料发酵及体外消化率的影响
DOI：
10.3168/jds.s0022-0302(89)79376-0
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发表时间：
1989
影响因子：
3.5
通讯作者：
K. A. Loney
中科院分区：
文献类型：
--
作者：
J. Harrison;S. Soderlund;K. A. Loney
以禾本科牧草为原料，在57 d的青贮期内，研究了不同接种量对乳酸菌发酵及体外消化率的影响。将切碎的饲料（DM = 28%）以4至6-kg的量青贮，并处理为：1）对照，10（3）附生乳酸菌; 2）10（5）添加乳酸菌;和3）10（6）添加乳酸菌/g湿饲料。在发酵第0、1、2、3、6、10、14、29和57天采集样品进行分析。观察到经处理的青贮饲料具有：1）更大量的乳酸菌，2）更大比例的同型发酵乳酸菌，和3）具有更大生物活性的乳酸菌。添加各数量的乳酸菌：1）提高了水溶性碳水化合物的利用率，降低了pH值; 2）限制了NH3-N的形成; 3）提高了DM和ADF的体外消化率。发酵57 d后，青贮饲料的乳酸含量无差异。
Grass-legume forage was used to evaluate the effect of inoculation rate of selected strains of lactic acid bacteria on fermentation and in vitro digestibility during 57 d of ensiling. Chopped forage (DM = 28%) was ensiled in 4 to 6-kg quantities and treated as: 1) control, 10(3) epiphytic lactic acid bacteria; 2) 10(5) added lactic acid bacteria; and 3) 10(6) added lactic bacteria/g of wet forage. Samples were obtained for analyses on d 0, 1, 2, 3, 6, 10, 14, 29, and 57 of fermentation. Treated silages were observed to have: 1) greater quantities of lactic acid bacteria, 2) a greater proportion of homofermentative lactic acid bacteria, and 3) lactic acid bacteria with greater biological activity. Addition of each amount of lactic acid bacteria: 1) increased the rate of utilization of water-soluble carbohydrate and decline in pH, 2) limited the formation of NH3 N, and 3) increased the in vitro digestibility of DM and ADF. No differences were observed in the lactic acid content of the silages after 57 d of fermentation.