Novel Loci for Kernel Hardness Appeared as a Response to Heat and Combined Heat-Drought Conditions in Wheat Harboring Aegilops tauschii Diversity
Novel Loci for Kernel Hardness Appeared as a Response to Heat and Combined Heat-Drought Conditions in Wheat Harboring Aegilops tauschii Diversity
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DOI：
10.3390/agronomy11061061
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发表时间：
2021-06-01
期刊：
影响因子：
3.7
通讯作者：
Tsujimoto, Hisashi
中科院分区：
文献类型：
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作者：
Elhadi, Gamila Mohamed Idris;Kamal, Nasrein Mohamed;Tsujimoto, Hisashi
籽粒硬度影响小麦的制粉和烘烤品质。高温和高温干旱等胁迫环境会使籽粒变硬，从而影响小麦的整体品质。除了已知决定硬度的嘌呤多啉基因外，其他qtl也决定硬度。这些qtl，特别是在逆境条件下，需要广泛的研究。此外，了解应力条件下硬度的变化或稳定以及与应力公差的关系将有助于选择即使在应力环境下也能保持高产量和高质量的优良品种。因此，在当前的工作中，我们的目的是确定遗传位点和标记性状关联（mta），这些遗传位点和标记性状关联（mta）有助于日本在最佳条件下的硬度，以及苏丹的高温和高温干旱（HD）条件下的硬度。我们使用了多种合成衍生物（MSD），具有不同的陶氏Aegilops基因组片段，并研究了硬度稳定与应力耐受性之间的关系。在胁迫条件下，我们观察到籽粒重量减少较少与籽粒硬度变化低或稳定有关。在所有条件下，我们鉴定了47个与硬度相关的标记；D基因组是主要贡献者。我们首次在4D染色体上发现了应力条件下硬度的显著相关性。我们剖析了与应力条件下硬度变化相关的几个候选基因。本研究结果将有助于进一步了解影响小麦硬度稳定性的遗传因素。
Kernel hardness influences the milling and baking quality of wheat. Stress environments such as heat and combined heat-drought can produce harder kernels, thereby affecting the overall wheat quality. Beside puroindoline genes that are known to determine hardness, other QTLs contribute to the hardness. These QTLs, especially under stress conditions, need extensive research. Moreover, understanding the modification or stabilization of hardness under stress condition and the relationship with stress tolerance will facilitate the selection of superior lines that maintain both high yield and quality even under the stress environment. Therefore, in the current work, we aimed to identify the genetic loci and marker trait associations (MTAs) that contributes for hardness under optimum conditions in Japan, and heat and combined heat-drought (HD) conditions in Sudan. We used a panel of multiple synthetic derivatives (MSD) having diverse Aegilops tauschii genome segments and investigated the association between hardness stabilization and stress tolerance. Under stress conditions, we observed that less reduction of kernel weight is associated with either low change or stable kernel hardness. We identified 47 markers associated with hardness under all conditions; the D genome was the main contributor. For the first time, we found a significant association with hardness under stress conditions on chromosome 4D. We dissected several candidate genes associated with the change of hardness under stress conditions. Our results will improve the understanding of the genetic factors that affect wheat hardness stability.