Quantitative MALDI-MS/MS assay for serum cortisol through charged derivatization
Quantitative MALDI-MS/MS assay for serum cortisol through charged derivatization
复制标题
通过带电衍生化定量 MALDI-MS/MS 测定血清皮质醇
DOI：
10.1016/j.jpba.2019.112912
复制
发表时间：
2020
影响因子：
3.4
通讯作者：
Higashi Tatsuya
中科院分区：
文献类型：
--
作者：
Nakamura Masahiro;Moritsuna Marina;Yuda Keita;Fujimura Shunsuke;Sugiura Yuki;Shimma Shuichi;Nishimoto Koshiro;Nishikawa Tetsuo;Suematsu Makoto;Ogawa Shoujiro;Higashi Tatsuya
皮质醇（Cortisol，CRT）是人体内主要的糖皮质激素，在许多生理过程中起着至关重要的作用，因此，测定其血清水平具有重要的临床意义。基质辅助激光解吸/电离-串联质谱（MALDI-MS/MS）可能是一种有效的方法，用于定量血清中的CRT，由于其有吸引力的性质，即，特异性强、使用方便、耐用、分析快速。在这项研究中，我们开发了一种方法来定量血清CRT的MALDI-MS/MS。这种方法采用的衍生化使用的Girard型试剂，1-（肼基羰基甲基）异喹啉氯化物，这弥补了缺乏灵敏度。该方法能够使用20 μL样品对血清CRT进行重现性定量（试验内和试验间相对标准偏差为7.4%或更低），可测量范围为25-500 ng/mL。用新建立的MALDI-MS/MS法测定的血清CRT浓度与液相色谱/电喷雾电离-MS/MS或电化学发光免疫分析法测定的结果一致。将MALDI-MS/MS方法用于正常人外周静脉血清和原发性醛固酮增多症患者肾上腺静脉血清的分析，获得了满意的结果。
Cortisol (CRT), the main glucocorticoid in humans, plays a crucial role in many physiological processes, therefore, the measurement of its serum level is of great clinical significance. Matrix-assisted laser desorption/ionization-tandem mass spectrometry (MALDI-MS/MS) might be an effective approach for the quantification of CRT in serum due to its attractive properties,i.e., high specificity, ease of use, ruggedness and rapid analysis. In this study, we developed a method to quantify the serum CRT by MALDI-MS/MS. This method employed the derivatization using a Girard-type reagent, 1-(hydrazinocarbonylmethyl)isoquinolinium chloride, which compensated for the lack of sensitivity. This method enabled the reproducible quantification of the serum CRT using a 20-μL sample (intra- and inter-assay relative standard deviations, 7.4% or lower), and the measurable range was 25–500 ng/mL. The serum CRT concentrations determined by the newly-developed MALDI-MS/MS method agreed well with those by liquid chromatography/electrospray ionization-MS/MS or electrochemiluminescence immunoassay. The MALDI-MS/MS method was used for the analysis of peripheral venous serum samples of healthy subjects and adrenal venous serum samples of patients with primary aldosteronism, and satisfactory results were obtained.