Large-scale gene expression profiling of discrete brain regions: Potential, limitations, and application in genetics of aggressive behavior
Large-scale gene expression profiling of discrete brain regions: Potential, limitations, and application in genetics of aggressive behavior
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DOI：
10.1023/a:1025726801047
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发表时间：
2003-09-01
影响因子：
2.6
通讯作者：
Datson, NA
中科院分区：
文献类型：
--
作者：
Feldker, DEM;de Kloet, ER;Datson, NA
许多行为遗传学家对揭开攻击性行为背后的分子机制感兴趣。到目前为止，大多数科学家对攻击性相关基因的研究都建立在一个初步的功能假设之上。大规模的基因表达谱技术，如基因表达序列分析(SAGE)和DNA微阵列，现在可以同时筛选数千个基因的表达水平，从而能够识别在大脑中表达的新的候选攻击相关基因，从而产生新的假设。然而，大脑的表达谱是具有挑战性的，因为大脑是一个复杂的异质组织，其中有大量的基因表达，基因表达的变化相对较小。在本期特刊中，我们重点介绍SAGE和DNA微阵列的原理以及它们的优缺点，并将其应用于脑组织中的分析，以期识别攻击相关基因。
Many behavioral geneticists are interested in unraveling the molecular mechanisms underlying aggressive behavior. So far, most scientists have based their search for aggression-related genes on a preliminary functional hypothesis. Large-scale gene expression profiling techniques, such as serial analysis of gene expression ( SAGE) and DNA microarrays, now enable the screening of expression levels of thousands of genes simultaneously, allowing the identification of new candidate aggression-related genes expressed in brain and thus the generation of new hypotheses. However, expression profiling in the brain is challenging, as brain is a complex heterogeneous tissue where large numbers of genes are expressed and relatively small changes in gene expression occur. In this special issue, we focus on the principles of SAGE and DNA microarrays, as well as their advantages and disadvantages and application to analysis in brain tissue in order to identify aggression-related genes.