Fully implantable, battery-free wireless optoelectronic devices for spinal optogenetics.
Fully implantable, battery-free wireless optoelectronic devices for spinal optogenetics.
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DOI：
10.1097/j.pain.0000000000000968
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发表时间：
2017-11
期刊：
影响因子：
7.4
通讯作者：
Gereau RW 4th
中科院分区：
文献类型：
--
作者：
Samineni VK;Yoon J;Crawford KE;Jeong YR;McKenzie KC;Shin G;Xie Z;Sundaram SS;Li Y;Yang MY;Kim J;Wu D;Xue Y;Feng X;Huang Y;Mickle AD;Banks A;Ha JS;Golden JP;Rogers JA;Gereau RW 4th
光遗传学工具的出现使人们对神经网络的组织有了前所未有的了解。虽然最近开发的技术已经实现了光遗传学在自由移动的无束缚动物中的脑功能研究，但无线供电和设备耐用性在脊髓回路的研究中提出了挑战，其中对周围硬组织和软组织的动态多维运动可能导致设备退化。我们在这里展示了一种由近场无线通信技术供电的完全可植入的光电设备，具有薄而灵活的开放式架构，可提供出色的机械耐用性，针对生物流体渗透的坚固密封和无线激活的保真度，从而允许对脊髓进行长期的光学刺激，而不会限制动物的自然行为。该系统由一个双层，矩形形状的磁性线圈天线连接到一个微型无机发光二极管（m-LED）上的薄，灵活的探针，可以被植入小鼠脊髓硬脑膜上方的有效刺激的光敏感蛋白在背角的神经元表达。用脊髓m-ILED无线光遗传学激活TRPV 1-ChR 2传入神经，在动物中持续操作数周至数月，引起伤害性行为和强烈的实时位置厌恶。控制系统所需的相对低成本的电子设备，以及这些设备的生物相容性和稳健的操作，将允许光遗传学在未来的脊髓回路研究中的广泛应用，以及在清醒、自由移动和不受束缚的动物中的各种外周靶点，其中现有的方法具有有限的实用性。
The advent of optogenetic tools has allowed unprecedented insights into the organization of neuronal networks. Although recently developed technologies have enabled implementation of optogenetics for studies of brain function in freely moving, untethered animals, wireless powering and device durability pose challenges in studies of spinal cord circuits where dynamic, multidimensional motions against hard and soft surrounding tissues can lead to device degradation. We demonstrate here a fully implantable optoelectronic device powered by near-field wireless communication technology, with a thin and flexible open architecture that provides excellent mechanical durability, robust sealing against biofluid penetration and fidelity in wireless activation, thereby allowing for long-term optical stimulation of the spinal cord without constraint on the natural behaviors of the animals. The system consists of a double-layer, rectangular-shaped magnetic coil antenna connected to a microscale inorganic light-emitting diode (m-ILED) on a thin, flexible probe that can be implanted just above the dura of the mouse spinal cord for effective stimulation of light-sensitive proteins expressed in neurons in the dorsal horn. Wireless optogenetic activation of TRPV1-ChR2 afferents with spinal m-ILEDs causes nocifensive behaviors and robust real-time place aversion with sustained operation in animals over periods of several weeks to months. The relatively low-cost electronics required for control of the systems, together with the biocompatibility and robust operation of these devices will allow broad application of optogenetics in future studies of spinal circuits, as well as various peripheral targets, in awake, freely moving and untethered animals, where existing approaches have limited utility.
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影响因子：
13.9
作者：
Fenno L;Yizhar O;Deisseroth K
通讯作者：
Deisseroth K
影响因子：
25
作者：
通讯作者：
--
DOI：
10.1126/science.1232437
发表时间：
2013-04-12
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Kim TI;McCall JG;Jung YH;Huang X;Siuda ER;Li Y;Song J;Song YM;Pao HA;Kim RH;Lu C;Lee SD;Song IS;Shin G;Al-Hasani R;Kim S;Tan MP;Huang Y;Omenetto FG;Rogers JA;Bruchas MR
通讯作者：
Bruchas MR
影响因子：
48
作者：
Farrar, Matthew J.;Bernstein, Ida M.;Schlafer, Donald H.;Cleland, Thomas A.;Fetcho, Joseph R.;Schaffer, Chris B.
通讯作者：
Schaffer, Chris B.
影响因子：
7.4
作者：
Copits BA;Pullen MY;Gereau RW 4th
通讯作者：
Gereau RW 4th