Heterogeneity of Leishmania donovani Parasites Complicates Diagnosis of Visceral Leishmaniasis: Comparison of Different Serological Tests in Three Endemic Regions
Heterogeneity of Leishmania donovani Parasites Complicates Diagnosis of Visceral Leishmaniasis: Comparison of Different Serological Tests in Three Endemic Regions
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DOI：
10.1371/journal.pone.0116408
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发表时间：
2015-03-03
期刊：
影响因子：
3.7
通讯作者：
Steinhoff, Ulrich
中科院分区：
文献类型：
--
作者：
Abass, Elfadil;Kang, Cholho;Steinhoff, Ulrich
基于单一利什曼原虫菌株抗原的内脏利什曼病诊断测试在异源寄生虫占主导地位的地区可能具有较低的诊断性能。本研究的目的是调查和比较三个内脏利什曼病流行国家基于不同利什曼原虫抗原的五种血清学检测的性能。通过以下血清学检测对来自苏丹东部、印度北部和法国南部三个内脏利什曼病流行地区的有症状和无症状病例和对照的总共 231 份血清进行了评估：rKLO8 和 rK39 ELISA、DAT (ITMA-DAT) 以及 rK39 (IT LEISH) 和 rKE16 (Signal-KA) 的两项快速检测。总体而言，rKLO8-和 rK39 ELISA 在所有流行地区的免疫功能正常的患者中最为敏感 (96-100%)，而在来自法国的 HIV 合并感染患者中，敏感性降低至 81.8%。与来自苏丹的血清相比，来自印度的患者血清对 rKLO8 和 rK39 的抗体反应明显更高（p
Diagnostic tests for visceral leishmaniasis that are based on antigens of a single Leishmania strain can have low diagnostic performance in regions where heterologous parasites predominate. The aim of this study was to investigate and compare the performance of five serological tests, based on different Leishmania antigens, in three endemic countries for visceral leishmaniasis. A total number of 231 sera of symptomatic and asymptomatic cases and controls from three endemic regions of visceral leishmaniasis in East Sudan, North India and South France were evaluated by following serological tests: rKLO8-and rK39 ELISA, DAT (ITMA-DAT) and two rapid tests of rK39 (IT LEISH) and rKE16 (Signal-KA). Overall, rKLO8-and rK39 ELISA were most sensitive in immunocompetent patients from all endemic regions (96-100%) and the sensitivity was reduced to 81.8% in HIV co-infected patients from France. Sera of patients from India demonstrated significantly higher antibody responses to rKLO8 and rK39 compared with sera from Sudan (p