High-affinity, protective antibodies to the binding domain of botulinum neurotoxin type A
High-affinity, protective antibodies to the binding domain of botulinum neurotoxin type A
复制标题
DOI：
10.1128/iai.69.1.570-574.2001
复制
发表时间：
2001-01-01
影响因子：
3.1
通讯作者：
Bavari, S
中科院分区：
文献类型：
--
作者：
Pless, DD;Torres, ER;Bavari, S
针对肉毒杆菌神经毒素A（BoNT/A）的推定结合结构域（无毒的50-kDa片段）制备单克隆抗体（MAbs）。最初，针对全毒素BoNT/A和针对结合片段BoNT/AH-C筛选所有融合产物。克隆了11个中和性杂交瘤，并通过表面等离子体共振证明了它们与BoNT/AH-C的特异性结合，解离常数范围为0.9至2.0。
Monoclonal antibodies (MAbs) were prepared against the putative binding domain of botulinum neurotoxin A (BoNT/A), a nontoxic 50-kDa fragment. Initially, all fusion products were screened against the holotoxin BoNT/A and against the binding fragment, BoNT/A H-C. Eleven neutralizing hybridomas were cloned, and their specific binding to BoNT/A H-C was demonstrated by surface plasmon resonance, with dissociation constants ranging from 0.9 to