Measurement of the deformation of isolated chondrocytes in agarose subjected to cyclic compression
Measurement of the deformation of isolated chondrocytes in agarose subjected to cyclic compression
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DOI：
10.1016/s1350-4533(98)00080-0
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发表时间：
1998-11-01
影响因子：
2.2
通讯作者：
Bader, DL
中科院分区：
文献类型：
--
作者：
Knight, MM;Ghori, SA;Bader, DL
机械诱导的细胞变形是许多可能的机械转导途径之一，通过这种途径，软骨细胞感知和响应其机械环境的变化。本研究描述了一种测量琼脂糖中分离软骨细胞在静态和循环压缩期间变形的系统。安装在倒置显微镜台上的测试平台用于对浸泡在培养基中的单个细胞琼脂糖构建体施加精确水平的压缩应变。使用连接在显微镜上的CCD摄像机记录细胞图像。细胞变形用变形指数（X/Y）来量化，该指数代表细胞直径与压缩轴平行(X)和垂直（IT）的比值。0 ~ 15%应变的循环压缩，在0.3 Hz下，导致相同频率的细胞循环变形。然而，在非应变阶段，细胞并没有完全恢复到最初的球形形态（X/Y = 1.0）。在应变阶段，变形水平（X/Y = 0.59）最初与静态15%应变时观察到的水平相似。然而，在20分钟的循环压缩期间（X/Y = 0.72），这种细胞变形水平降低，尽管在静态压缩期间细胞变形保持不变。该系统可用于检查在一系列动态机械刺激下的细胞事件。(c) 1999年方案。Elsevier Science Ltd.出版。版权所有。
Mechanically induced cell deformation is one of a number of possible mechanotransduction pathways by which chondrocytes sense and respond to changes in their mechanical environment. The present study describes a system for measuring the deformation of isolated chondrocytes in agarose during both static and cyclic compression. A test rig mounted on the stage of an inverted microscope was used to apply precise levels of compressive strain to individual cell-agarose constructs bathed in culture medium. images of the cells were recorded using a CCD video camera attached to the microscope. Cell deformation was quantified in terms of a deformation index (X/Y) representing the ratio of cell diameters measured parallel (X) and perpendicular (IT) to the axis of compression. Cyclic compression between 0 and 15% strain, at 0.3 Hz, resulted in cyclic deformation of the cells at the same frequency. However, during the unstrained phase the cells did not fully recover to their initially spherical morphology (X/Y = 1.0). During the strained phase, the level of deformation (X/Y = 0.59) was initially similar to that observed during static 15% strain. However, this level of cell deformation reduced over a 20 min period of cyclic compression (X/Y = 0.72), although during static compression the cell deformation remained constant. This system may be used to examine cellular events under a range of dynamic mechanical stimuli. (C) 1999 IPEM. Published by Elsevier Science Ltd. All rights reserved.