Differences in Hsp70 expression in the sporozoites of the original strain and precocious lines of Eimeria tenella
Differences in Hsp70 expression in the sporozoites of the original strain and precocious lines of Eimeria tenella
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DOI：
10.1645/ge-3464.1
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发表时间：
2005-10-01
影响因子：
1.3
通讯作者：
Sánchez-Acedo, C
中科院分区：
文献类型：
--
作者：
del Cacho, E;Gallego, M;Sánchez-Acedo, C
比较了一株野生型亲本菌株和2株柔嫩艾美耳球虫早熟系孢子子中热休克蛋白70 （hsp70）的表达水平，探讨了热休克蛋白表达与菌株毒力的关系。通过免疫组织化学技术、免疫印迹和流式细胞术分析Hsp70在孢子体中的表达。鉴定了一条70 kDa的条带，并通过光学密度分析量化了Hsp70表达水平的变化。结果显示，随着衰减的进行，Hsp70在线虫孢子子中的表达逐渐显著降低，提示出囊线虫孢子子中表达的Hsp70可能参与了疟原虫的致病性。此外，在野生菌株的整个孢子体中观察到的Hsp70的细胞质分布在早熟系中减少到前面部分。
The levels of expression of Heat shock protein 70 (Hsp 70) in sporozoites of a wild-type parent strain and 2 precocious lines of Eimeria tenella, were compared to investigate the relationship between the heat shock proteins expressed by the parasite and virulence of the strain. Hsp70 expression was analyzed in sporozoites by immunohistochemical techniques, immunoblot, and flow cytometric analyses. One band of 70 kDa was identified and the variation of the Hsp70 expression levels was quantified by optical densitometric analyses. The results showed a significant gradual decrease in the Hsp70 expression in sporozoites of E. tenella as attenuation progressed, suggesting that the Hsp70 expressed in the excysted sporozoites of E. tenella might be involved in parasite pathogenicity. In addition, the cytoplasmic distribution of the Hsp70, which was observed in the entire sporozoites of the wild strain, was reduced to the anterior portion in the precocious lines.