Synthesis of near-infrared absorbing triangular Au nanoplates using biomineralisation peptides.
Synthesis of near-infrared absorbing triangular Au nanoplates using biomineralisation peptides.
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使用生物矿化肽合成近红外吸收三角形金纳米板。
DOI：
10.1016/j.actbio.2021.06.010
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发表时间：
2021
影响因子：
9.7
通讯作者：
Tanaka M
中科院分区：
文献类型：
--
作者：
Tanaka M
三角形Au纳米板(TrAuNPls)具有很强的等离子体性质，可作为光热剂用于癌症治疗。然而，这种形态的受控制备通常需要苛刻的合成条件。生物分子为开发生物相容的合成方案提供了另一条途径。特别是，多肽为环境条件下的无机合成提供了一条新的途径。本文以预先分离的Au纳米粒子(AuNP)合成肽ASHQWAWKWE为基础，研究了在室温下通过HAuCl4和多肽一锅合成法制备TrAuNPls的条件，以有效地获得具有近红外吸收的近红外吸收粒子，用于非侵入性光学诊断和光疗。通过调节多肽浓度，可在中性pH条件下控制TrAuNPls的大小和性质。所合成的微粒在体外具有作为光热治疗剂的潜力。此外，使用B3衍生物的多肽表征揭示了第三个氨基酸组氨酸在形态调节和潜在的环状Au纳米板(AuNPl)合成中的重要性。这些发现不仅为TrAuNpls和环状AuNpls提供了一种简单、绿色的合成方法，而且有助于阐明基于多肽的纳米粒子合成在癌症治疗中的调节作用。意义陈述生物分子作为一种载体，在环境条件下合成具有特定性质的无机材料越来越受到关注，特别是短肽有可能控制具有定制功能的纳米材料的合成。在这里，评价了一种先前分离的多肽在合成含有具有近红外吸收的十面体和三角形纳米片的金纳米颗粒中的应用。观察到的三角形纳米板的大小和吸收峰与多肽浓度有关。此外，这些微调的三角形纳米板显示了作为光疗剂的潜力。此外，这些多肽衍生物还表明了合成圆形纳米平板的可能性。这些发现可能为开发利用无毒分子在温和的条件下合成具有生物应用的功能纳米颗粒提供新的技术，原始的B3肽被强调。
Triangular Au nanoplates (TrAuNPls) possessing strong plasmonic properties can be used as photothermal agents in cancer therapy. However, the controlled preparation of such morphologies typically requires harsh synthetic conditions. Biomolecules offer an alternative route to developing biocompatible synthetic protocols. In particular, peptides offer a novel route for inorganic synthesis under ambient conditions. Herein, using the previously isolated peptide, ASHQWAWKWE, for Au nanoparticle (AuNP) synthesis, the conditions for preparing TrAuNPls via a one-pot synthetic process of mixing HAuCl4and peptides at room temperature were investigated to effectively obtain particles possessing near-infrared absorbance for non-invasive optical diagnosis and phototherapy. By adjusting the peptide concentration, the size and property of TrAuNPls were controlled under neutral pH conditions. The synthesised particles showed potential as photothermal therapeutic agentsin vitro. In addition, peptide characterisation using B3 derivatives revealed the importance of the third amino acid histidine in morphological regulation and potential circular Au nanoplates (AuNPl) synthesis with ASEQWAWKWE and ASAQWAWKWE peptides. These findings provide not only an easy and green synthetic method for TrAuNPls and circular AuNPls, but also some insight to help elucidate the regulation of peptide-based nanoparticle synthesis for use in cancer therapy.Statement of significanceBiological molecules have received increasing attention as a vehicle to synthesise inorganic materials with specific properties under ambient conditions; particularly, short peptides have the potential to control the synthesis of nanoscale materials with tailored functions. Here, the application of a previously isolated peptide was assessed in synthesising Au nanoparticles containing decahedral and triangular nanoplates with near-infrared absorbance. The size and absorbance peaks of the triangular nanoplates observed were peptide concentration-dependent. In addition, these fine-tuned triangular nanoplates exhibited potential as a phototherapeutic agent. Moreover, the peptide derivatives indicated the possibility of synthesising circular nanoplates. These findings may offer insight into development of new techniques for synthesising functional nanoparticles having biological applications using non-toxic molecules under mild conditions stituted in the original B3 peptide is underlined.