Increases in 13-hydroxyoctadecadienoic acid dehydrogenase activity during differentiation of cultured cells.
Increases in 13-hydroxyoctadecadienoic acid dehydrogenase activity during differentiation of cultured cells.
复制标题
培养细胞分化过程中 13-羟基十八二烯酸脱氢酶活性增加。
DOI：
10.1093/carcin/14.11.2239
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发表时间：
1993
期刊：
影响因子：
4.7
通讯作者：
J. Rafter
中科院分区：
文献类型：
--
作者：
A. Bull;C. Branting;J. Bronstein;M. Blackburn;J. Rafter
最近，亚油酸的氧化产物如 13-羟基十八碳二烯酸 (HODE) 与细胞生理学的调节有关，包括对生长因子治疗的增殖反应。此外，最近还描述了一种 NAD(+) 依赖性 13-HODE 脱氢酶。为了评估这种酶对细胞过程的贡献，我们检查了该酶在不同条件下的行为。在本报告中，检查了两种不同细胞系体外分化期间13-羟基十八碳二烯酸脱氢酶活性的变化。细胞系 HT-29 通过操纵培养基进行诱导分化，而 Caco-2 细胞系在达到汇合后进行自发分化。在这两种细胞系中，较长的培养时间都伴随着 13-HODE 脱氢酶活性的增加。即使细胞增殖停止后，酶活性仍继续增加。通过观察蔗糖酶和碱性磷酸酶活性的增加来验证细胞分化。此外，在未分化的瑞士小鼠 3T3 成纤维细胞的生长、早期汇合和晚期汇合培养物中测量了 13-HODE 脱氢酶的活性。在成纤维细胞系中，在实验过程中没有观察到13-HODE脱氢酶活性的显着变化。 13-HODE脱氢酶的比活性在三种细胞系之间也存在显着差异，这与分化程度一致。 Caco-2 细胞中的活性水平最高（200-400 pmol/min/mg），成纤维细胞中的活性水平几乎检测不到（0.6-2 pmol/min/mg）。 13-HODE 脱氢酶与细胞分化之间的相关性表明该酶可能在细胞增殖和分化途径之间的分配中发挥作用。
Recently, oxidation products of linoleic acid such as 13-hydroxyoctadecadienoic acid (HODE) have been implicated in the regulation of cellular physiology including the proliferative response to growth factor treatment. In addition, an NAD(+)-dependent 13-HODE dehydrogenase was recently described. To evaluate the contribution of this enzyme to cellular processes we have examined the behavior of the enzyme under different conditions. In the present report, changes in the activity of 13-hydroxyoctadecadienoic acid dehydrogenase during in vitro differentiation of two different cell lines were examined. The cell line HT-29 undergoes induced differentiation via manipulation of the medium while the Caco-2 line undergoes spontaneous differentiation upon attainment of confluence. In both cell lines, longer culture times were accompanied by increases in 13-HODE dehydrogenase activity. The increase in enzyme activity continued even after cell proliferation had ceased. Cellular differentiation was verified by the observation of increases in sucrase and alkaline phosphatase activities. In addition, the activity of 13-HODE dehydrogenase was measured in growing, early confluent and late confluent cultures of undifferentiating Swiss mouse 3T3 fibroblasts. In the fibroblast line, no significant changes in 13-HODE dehydrogenase activity were observed during the course of the experiment. The specific activity of 13-HODE dehydrogenase was also significantly different between the three cell lines, consistent with the extent of differentiation. Highest levels of activity were found in Caco-2 cells (200-400 pmol/min/mg) and barely detectable levels in the fibroblasts (0.6-2 pmol/min/mg). The correlation between 13-HODE dehydrogenase and cell differentiation suggests the enzyme may have a role to play in the partitioning of cells between proliferation and differentiation pathways.