NMR scaler couplings across Watson-Crick base pair hydrogen bonds in DNA observed by transverse relaxation optimized spectroscopy
NMR scaler couplings across Watson-Crick base pair hydrogen bonds in DNA observed by transverse relaxation optimized spectroscopy
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DOI：
10.1073/pnas.95.24.14147
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发表时间：
1998-11-24
影响因子：
11.1
通讯作者：
Wüthrich, K
中科院分区：
文献类型：
--
作者：
Pervushin, K;Ono, A;Wüthrich, K
本文描述了DNA双链(h)J（NN）和(h)J（HN）中N-15-N-15和h -1- n- 15碱基对氢键上的标量偶联的核磁共振观察。这些偶联为DNA的结构研究和氢键性质的理论研究提供了新的参数。采用二维[N-15， h -1]-横向弛豫优化光谱（TROSY），利用N-15标记的14-mer DNA双工测量了6-7 Hz范围内的(h)J(NN)，利用二维(h)J(NN)-相关-[N-15， h -1]-TROSY实验，对与氢键相关的N-15自旋对的化学位移进行了关联，并首次观察到(h)J（HN）标量耦合，其值在2-3.6 Hz范围内。基于trosy的跨氢键的标量偶联研究应该适用于大分子大小，包括蛋白质结合的核酸。
This paper describes the NMR observation of N-15-N-15 and H-1-N-15 scalar couplings across the hydrogen bonds in Watson-Crick base pairs in a DNA duplex, (h)J(NN) and (h)J(HN). These couplings represent new parameters of interest for both structural studies of DNA and theoretical investigations into the nature of the hydrogen bonds. Two dimensional [N-15,H-1]-transverse relaxation-optimized spectroscopy (TROSY) with a N-15-labeled 14-mer DNA duplex was used to measure (h)J(NN), which is in the range 6-7 Hz, and the two-dimensional (h)J(NN)-correlation-[N-15,H-1]-TROSY experiment was used to correlate the chemical shifts of pairs of hydrogen bond-related N-15 spins and to observe, for the first time, (h)J(HN) scalar couplings, with values in the range 2-3.6 Hz, TROSY-based studies of scalar couplings across hydrogen bonds should be applicable for large molecular sizes, including protein-bound nucleic acids.