Abundance and Significance of Neuroligin-1 and Neurexin II in the Enteric Nervous System of Embryonic Rats.
Abundance and Significance of Neuroligin-1 and Neurexin II in the Enteric Nervous System of Embryonic Rats.
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Neuroligin-1 和 Neurexin II 在胚胎大鼠肠神经系统中的丰度及意义
DOI：
10.1155/2017/1209360
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发表时间：
2017
影响因子：
--
通讯作者：
Li A
中科院分区：
文献类型：
--
作者：
Wang D;Pan J;Song G;Gao N;Zheng Y;Zhang Q;Li A
目的。探讨不同胚胎日大鼠肠神经系统（ENS）中neuroligin-1和neurexin II的丰度并探讨其潜在意义。方法。分别研究了胚胎第16、18和20天的大鼠以及24小时内的新生儿（E16、E18、E20和Ep0）的近回盲部连接处的全层结肠标本。采用qRT-PCR检测Neuroligin-1和Neurexin II mRNA的表达，并采用Western blotting进一步检测其在整个组织中的表达。最后，使用免疫组织化学染色阐明了 Neuroligin-1 和 neurexin IIα 的组织学外观。结果。 qRT-PCR结果显示，E16、E18、E20、Ep0组神经胶质素1和神经毒素II mRNA表达量随着胚胎大鼠的生长逐渐升高（P < 0.05）。 Western blotting证实了增加的趋势。免疫组化染色显示，蛋白neuroligin-1和neurexin IIα阳性细胞主要集中在结肠肌间神经丛，其表达依赖于胚胎时间。结论。 Neuroligin-1 和 neurexin II 均在 ENS 中表达，并且与 ENS 的发育具有时间相关性，在此期间，由于它们的破坏和随之而来的异常 ENS 发育，可能会发生神经元肠畸形 (NIM)。
Aim. To investigate the abundance of neuroligin-1 and neurexin II in the enteric nervous system (ENS) of rats on different embryonic days and to explore their potential significance. Methods. The full-thickness colon specimens proximal to the ileocecal junction of rats on embryonic days 16, 18, and 20 and of newborns within 24 hours (E16, E18, E20, and Ep0) were studied, respectively. qRT-PCR was applied for detecting the expressions of neuroligin-1 and neurexin II on mRNA, and western blotting was employed for detecting their further expressions on the whole tissue. Finally, the histological appearance of neuroligin-1 and neurexin IIα was elucidated using immunohistochemical staining. Results. qRT-PCR showed that the neuroligin-1 and neurexin II mRNA expressions of groups E16, E18, E20, and Ep0 increased gradually with the growth of embryonic rats (P < 0.05). Western blotting confirmed the increasing tendency. In immunohistochemical staining, proteins neuroligin-1 and neurexin IIα positive cells concentrated mostly in the myenteric nerve plexus of the colon and their expressions depend on the embryonic time. Conclusion. Neuroligin-1 and neurexin II were both expressed in the ENS and have temporal correlation with the development of ENS, during which neuronal intestinal malformations (NIM) may occur due to their disruptions and consequent abnormal ENS development.