New adenovirus species found in a patient presenting with gastroenteritis
New adenovirus species found in a patient presenting with gastroenteritis
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DOI：
10.1128/jvi.02650-06
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发表时间：
2007-06-01
影响因子：
5.4
通讯作者：
Schnurr, David P.
中科院分区：
文献类型：
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作者：
Jones, Morris Saffold, II;Harrach, Balazs;Schnurr, David P.
洛杉矶县公共卫生部门从胃肠炎爆发期间提交的五份粪便样本中的每一份中，在原代猴细胞中培养了一种身份不明的病原体。电子显微镜和腺病毒特异性单克隆抗体证实该物质是腺病毒。由于病毒滴度太低，不可能进行完整的血清分型。利用不依赖DNA酶序列的病毒核酸扩增方法，我们鉴定了几个与人腺病毒41（HAdV-41）和猿猴腺病毒1（SAdV-1）具有高度同源性的核苷酸序列。然而，使用抗SAdV-1血清，确定该病毒在血清学上与SAdV-1不同。基因组测序和系统发育分析证实，这种新的腺病毒与已知的人类腺病毒非常不同，它不仅是一种新类型，而且代表了一个新物种（人类腺病毒G）。在一项回顾性临床研究中，通过聚合酶链反应（PCR）在另一名来自另一次胃肠炎爆发的患者中检测到了这种新病毒。这项研究表明，HAAV-52 可能是引起病因不明的胃肠炎的多种病原体之一。
An unidentified agent was cultured in primary monkey cells at the Los Angeles County Public Health Department from each of five stool specimens submitted from an outbreak of gastroenteritis. Electron microscopy and an adenovirus-specific monoclonal antibody confirmed this agent to be an adenovirus. Since viral titers were too low, complete serotyping was not possible. Using the DNase-sequence-independent viral nucleic acid amplification method, we identified several nucleotide sequences with a high homology to human adenovirus 41 (HAdV-41) and simian adenovirus 1 (SAdV-1). However, using anti-SAdV-1 sera, it was determined that this virus was serologically different than SAdV-1. Genomic sequencing and phylogenetic analysis confirmed that this new adenovirus was so divergent from the known human adenoviruses that it was not only a new type but also represented a new species (human adenovirus G). In a retrospective clinical study, this new virus was detected by PCR in one additional patient from a separate gastroenteritis outbreak. This study suggests that HAAV-52 may be one of many agents causing gastroenteritis of unknown etiology.