Comparison of the second and third generation anti-cyclic citrullinated peptide antibody assays in the diagnosis of Japanese patients with rheumatoid arthritis
Comparison of the second and third generation anti-cyclic citrullinated peptide antibody assays in the diagnosis of Japanese patients with rheumatoid arthritis
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DOI：
10.1007/s00296-009-1336-2
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发表时间：
2011-05-01
影响因子：
4
通讯作者：
Yamanaka, Hisashi
中科院分区：
文献类型：
--
作者：
Shidara, Kumi;Inoue, Eisuke;Yamanaka, Hisashi
抗环瓜氨酸肽（CCP）抗体在类风湿关节炎（RA）的诊断，特别是早期诊断中的作用日益重要。本研究的目的是比较第二代和第三代抗CCP抗体试剂盒在日本RA患者中的诊断有用性。使用来自106名类风湿性关节炎（RA）患者和57名非RA患者的血清样品，用第二代（MESACUP CCP测试，医学和生物实验室）和第三代（QUANTA Lite CCP 3 IgG ELISA，Inova Diagnostics）试剂盒测量抗CCP抗体滴度。比较敏感性和特异性。第二代抗CCP（抗CCP 2）试剂盒的敏感性和特异性分别为88.7%和89.5%，第三代抗CCP（抗CCP 3）试剂盒的敏感性和特异性分别为91.5%和87.7%。受试者工作曲线下面积显示抗CCP 2和抗CCP 3具有相似的诊断性能。在日本RA患者中，抗CCP 3检测试剂盒的诊断性能与抗CCP 2检测试剂盒相当。
The anti-cyclic citrullinated peptide (CCP) antibody has become increasingly important in the diagnosis of rheumatoid arthritis (RA), especially for early diagnosis. The purpose of this study is to compare the diagnostic usefulness of the second and third generation anti-CCP antibody kits among Japanese patients with RA. Anti-CCP antibody titers were measured with the second generation (MESACUP CCP test, Medical and biological laboratories) and third generation (QUANTA Lite CCP3 IgG ELISA, Inova Diagnostics) kits using serum samples from 106 rheumatoid arthritis (RA) patients and 57 non-RA patients. Sensitivities and specificities were compared. The sensitivity and specificity of the second generation anti-CCP (anti-CCP2) kit were 88.7 and 89.5%, and those of the third generation anti-CCP (anti-CCP3) kit were 91.5 and 87.7%. Area under the receiver operating curve showed that anti-CCP2 and anti-CCP3 had similar diagnostic performances. Diagnostic performance of the anti-CCP3 assay was comparable with the anti-CCP2 assay in Japanese patients with RA.