Expression of adrenomedullin2/intermedin in human brain, heart, and kidney
Expression of adrenomedullin2/intermedin in human brain, heart, and kidney
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DOI：
10.1016/j.peptides.2007.01.018
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发表时间：
2007-05-01
期刊：
影响因子：
3
通讯作者：
Takahashi, Kazuhiro
中科院分区：
文献类型：
--
作者：
Morimoto, Ryo;Satoh, Fumitoshi;Takahashi, Kazuhiro
肾上腺髓质素2/intermedin (AM2/IMD) 是降钙素/降钙素基因相关肽(CGRP) 家族的新成员。在本研究中，我们开发了一种针对人类 AM2/IMD 的特异性放射免疫测定法。通过放射免疫分析和免疫细胞化学研究了尸检获得的人脑、垂体、心脏和肾脏中 AM2/IMD 的表达。通过放射免疫测定法在人脑（范围；0.163-1.495 pmol/g 湿重）、垂体（4.46 +/- 0.689 pmol/g 湿重，平均值 +/- S.E.M，n = 3）、左心室（0.251 +/- 0.0321 pmol/g 湿重，n = 4）、肾脏（3.49 +/- 1.18 pmol/g 湿重，n = S），以及从健康受试者获得的血浆（24.7 +/- 1.78 pmol/l，n = 3）。反相高效液相色谱显示人脑、肾和血浆提取物中的免疫反应性AM2/IMD在真正的AM2/IMD位置被洗脱。在脑组织和血浆中发现了早期洗脱的其他峰。免疫细胞化学显示，免疫反应性-AM2/IMD定位于下丘脑室旁核和视上核、垂体前叶和后叶、心肌细胞、心包脂肪细胞、心包静脉的血管内皮细胞、冠状动脉和肾小动脉的血管平滑肌细胞以及肾小管细胞。本研究显示 AM2/IMD 在中枢神经系统和心血管系统的各种类型细胞中表达，并提示 AM2/IMD 在这些系统中可能的（病理）生理作用。 (c) 2007 年，爱思唯尔公司出版。
Adrenomedullin2/intermedin (AM2/IMD) is a novel member of the calcitonin/calcitonin gene-related peptide (CGRP) family. In the present study, we developed a specific radioimmunoassay of human AM2/IMD. Expression of AM2/IMD was studied in the human brain, pituitary, heart and kidney obtained at autopsy by radioimmunoassay and immunocytochemistry. Immunoreactive-AM2/IMD was detected by radioimmunoassay in human brains (range; 0.163-1.495 pmol/g wet weight), pituitaries (4.46 +/- 0.689 pmol/g wet weight, mean +/- S.E.M, n = 3), left ventricles of hearts (0.251 +/- 0.0321 pmol/g wet weight, n = 4), kidneys (3.49 +/- 1.18 pmol/g wet weight, n = S), and plasma obtained at healthy subjects (24.7 +/- 1.78 pmol/l, n = 3). Reverse-phase high performance liquid chromatography showed that immunoreactive-AM2/IMD in human brain, kidney and plasma extracts were eluted in the position of authentic AM2/IMD. Additional peaks eluted earlier were found in the brain tissue and plasma. Immunocytochemistry showed that immunoreactive-AM2/IMD was localized in paraventricular and supraoptic nuclei of hypothalamus, anterior and posterior lobes of pituitary, cardiomyocytes, pericardial adipocytes, vascular endothelial cells of pericardial veins, and vascular smooth muscle cells of coronary arteries and renal arterioles as well as in renal tubular cells. The present study has shown expression of AM2/IMD in various types of cells in the central nervous system and the cardiovascular system, and suggested possible (patho)physiological roles of AM2/IMD in these systems. (c) 2007 Published by Elsevier Inc.