De novo formation of basal bodies in Naegleria gruberi:: regulation by phosphorylation
De novo formation of basal bodies in Naegleria gruberi:: regulation by phosphorylation
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DOI：
10.1083/jcb.200410052
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发表时间：
2005-06-06
影响因子：
7.8
通讯作者：
Lee, J
中科院分区：
文献类型：
--
作者：
Kim, HK;Kang, JG;Lee, J
格鲁氏耐格里虫基体从头形成之前，先短暂形成含有 γ-微管蛋白、中心周蛋白和肌球蛋白 II 复合物（GPM 复合物）的微管 (MT) 成核复合物。 GPM 复合物的 MT 成核活性在可见基体形成之前达到最大，然后迅速下降。 GPM 复合物的 MT 成核活性的调节是通过复合物的瞬时磷酸化来完成的。 T基体形成后去磷酸化的抑制导致多鞭毛的形成。 2D 凝胶电泳和蛋白质印迹显示 GPM 复合物的 MT 成核活性与复合物中过度磷酸化的 γ-微管蛋白的存在之间存在平行关系。这些数据表明，GPM 复合物对 MT 的成核先于基体从头形成，并且 GPM 复合物的 MT 成核活性的调节对于基体数量的调节至关重要。
The de novo formation of basal bodies in Naegleria gruberi was preceded by the transient formation of a microtubule (MT)-nucleating complex containing gamma-tubulin, pericentrin, and myosin II complex (GPM complex). The MT-nucleating activity of GPM complexes was maximal just before the formation of visible basal bodies and then rapidly decreased. The regulation of MT-nucleating activity of GPM complexes was accomplished by a transient phosphorylation of the complex. Inhibition of dephosphorylation after the formation of T basal bodies resulted in the formation of multiple flagella. 2D-gel electrophoresis and Western blotting showed a parallel relationship between the MT-nucleating activity of GPM complexes and the presence of hyperphosphorylated gamma-tubulin in the complexes. These data suggest that the nucleation of MTs by GPM complexes precedes the de novo formation of basal bodies and that the regulation of MT-nucleating activity of GPM complexes is essential to the regulation of basal body number.