HYDROGEN ION BUFFERS FOR BIOLOGICAL RESEARCH
HYDROGEN ION BUFFERS FOR BIOLOGICAL RESEARCH
复制标题
DOI：
10.1021/bi00866a011
复制
发表时间：
1966-01-01
期刊：
影响因子：
2.9
通讯作者：
SINGH, RMM
中科院分区：
文献类型：
--
作者：
GOOD, NE;WINGET, GD;SINGH, RMM
诺曼·E·古德，G.Douglas Winget，Wilhelmina温特，Thomas N.Connolly，Seikichi Izawa，和Raizada。辛格摘要：十二个新的或很少使用的氢离子BUF--两个两性离子，TV-三(羟甲基)-FERS，覆盖pKa=6.15-8.35范围内，已经制备并测试了甲氨基乙磺酸和TV-2-羟基。10个是两性氨基酸，乙基哌嗪-TV‘-2-乙磺酸，部分是TV-取代的牛磺酸或TV-取代的甘氨酸，特别是活性和稳定性的线粒体制剂，2个是阳离子初级脂肪胺。在无细胞细菌制剂中，两性离子缓冲液在Hill反应和大豆线粒体对琥珀酸的磷酸化-羟甲基氨基甲烷(Tris)或磷酸偶联氧化反应中优于三离子缓冲液。缓冲区。
Norman E. Good, G. Douglas Winget, Wilhelmina Winter, Thomas N. Connolly, Seikichi Izawa, and Raizada. M. Singh abstract: Twelve new or little used hydrogen ion buf- Two of the zwitterions, TV-tris (hydroxymethyl)-fers covering the range pKa= 6.15-8 35 have been methylaminoethanesulfonic acid and TV-2-hydroxyprepared and tested. Ten are zwitterionic amino acids, ethylpiperazine-TV'-2-ethanesuIfonic acid, give partieither TV-substituted taurines or TV-substituted glycines, cularly activeand stable mitochondrial preparations, and two are cationic primary aliphatic amines. All of These two also give higher rates of protein synthesis the zwitterionic buffers are better than conventional in cell-free bacterial preparations than do tris-buffers in the Hill reaction and in the phosphorylation-(hydroxymethyl) aminomethane (Tris) or phosphate coupled oxidation of succinate by bean mitochondria. buffers.