Pleiotrophin cellular localization in nerve regeneration after peripheral nerve injury
Pleiotrophin cellular localization in nerve regeneration after peripheral nerve injury
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DOI：
10.1369/jhc.4a6574.2005
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发表时间：
2005-08-01
影响因子：
3.2
通讯作者：
Courty, J
中科院分区：
文献类型：
--
作者：
Blondet, B;Carpentier, G;Courty, J
多效生长因子（PTN）是肝素结合生长因子家族的一员，在体外显示促有丝分裂活性并促进神经突生长。在体内，PTN在胚胎晚期和出生后早期广泛表达于沿着发育轴突的通路。虽然PTN基因表达水平在成年期非常低，但该基因的激活可能发生在损伤恢复期间，并且似乎在组织再生过程中起重要作用。在这项研究中，我们调查是否PTN参与了周围神经损伤的再生过程。参考本地化的荧光标记有髓轴突，我们开发了一个特定的计算机工具，共定位的荧光图像与相位对比图像。免疫组化分析显示PTN在远端神经节段的不同类型的非神经细胞中，包括雪旺细胞、巨噬细胞和内皮细胞，但不在轴突中。雪旺氏细胞PTN免疫反应，早在2天后损伤，而PTN阳性的巨噬细胞被发现1周后。在所有时间点，在内皮细胞中观察到强PTN免疫反应性。这些发现支持PTN参与周围神经损伤的适应性反应的观点。更好地了解它的贡献可能会提出新的策略，促进周围神经再生。
Pleiotrophin (PTN) is a member of the family of heparin-binding growth factors that displays mitogenic activities and promotes neurite outgrowth in vitro. In vivo, PTN is widely expressed along pathways of developing axons during the late embryonic and early postnatal period. Although the level of PTN gene expression is very low during adulthood, activation of the gene may occur during recovery from injury and seems to play an important role in tissue regeneration processes. In this study, we investigated whether PTN was involved in the regenerative process of injured peripheral nerves. To refer localization of the fluorescent markers to myelinated axons, we developed a specific computer tool for colocalization of fluorescence images with phase contrast images. Immunohistochemical analysis showed PTN in different types of nonneural cells in distal nerve segments, including Schwann cells, macrophages, and endothelial cells, but not in axons. Schwann cells exhibited PTN immunoreactivity as early as 2 days after injury, whereas PTN-positive macrophages were found 1 week later. Strong PTN immunoreactivity was noted in endothelial cells at all time points. These findings support the idea that PTN participates in the adaptive response to peripheral nerve injury. A better understanding of its contribution may suggest new strategies for enhancing peripheral nerve regeneration.