Tenascin expression in cancer cells and stroma of human breast cancer and its prognostic significance.
Tenascin expression in cancer cells and stroma of human breast cancer and its prognostic significance.
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发表时间：
1995-09
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通讯作者：
A. Ishihara;Toshimichi Yoshida;H. Tamaki;T. Sakakura
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作者：
A. Ishihara;Toshimichi Yoshida;H. Tamaki;T. Sakakura
使用抗tenascin (TN) RCB1的单克隆抗体对210例人乳腺癌的福尔马林固定石蜡包埋组织切片进行免疫组织化学检测。77例（36.7%）乳腺癌间质中检测到免疫反应性TN，其余133例（63.3%）阴性。77例中，12例（5.7%）癌细胞质染色阳性。阳性细胞常见于癌巢边缘靠近间质的部位。根据TN的染色模式，将乳腺癌病例分为癌细胞TN(+)/间质TN（+）、癌细胞(-)/间质TN（+）、癌细胞(-)/间质TN（-）三组。分析这些TN模式与肿瘤各种临床病理特征及患者转归的关系发现，与癌细胞(-)/间质TN（-）组相比，癌细胞TN(+)/间质TN（+）组淋巴结转移频率增加，转归异常差，癌细胞(-)/间质TN（+）组转移频率更高，转归较差。大多数癌细胞TN(+)/间质TN（+）为c-erbB-2阳性，雌激素受体阴性。此外，对新获得的乳腺癌组织的原位杂交表明，癌细胞和基质细胞都表达TN mRNA。这些结果表明，乳腺癌中TN既由癌上皮细胞产生，也由间质间充质细胞产生，癌细胞TN可能参与了肿瘤的扩散，导致患者预后不良。
Sections of formalin-fixed, paraffin-embedded tissues from 210 human breast cancers were immunohistochemically examined using the mAb against human tenascin (TN) RCB1. Immunoreactive TN was detected in the breast cancer stroma in 77 (36.7%) cases, whereas the remaining 133 (63.3%) were negative. Of the 77, 12 (5.7%) cases also showed positive staining in the carcinoma cell cytoplasm. The positive cells were often observed in the margin of the cancer nests at the site adjacent to the stroma. According to the staining pattern of TN, the breast cancer cases were classified into the three groups of cancer cell TN(+)/stromal TN(+), cancer cell(-)/stromal TN(+), and cancer cell(-)/stromal TN(-). Analysis of the relationship of these TN patterns with various clinicopathological characteristics of the tumors and the patient outcome revealed that, in comparison to the cancer cell(-)/stromal TN(-) group, the cancer cell TN(+)/stromal TN(+) group exhibited increased frequency of lymph node metastasis and exceptionally poor outcome, and the cancer cell(-)/stromal TN(+) group also showed more frequent metastasis and poorer outcome. Most of the cancer cell TN(+)/stromal TN(+) cases were c-erbB-2 positive and estrogen receptor negative. Furthermore, in situ hybridization of freshly obtained breast cancer tissues demonstrated that both cancer cells and stromal cells express TN mRNA. These results indicate that the TN in breast cancer is produced by cancer epithelial cells as well as by stromal mesenchymal cells, and that cancer cell TN might be involved in cancer spreading, resulting in unfavorable patient prognosis.