Local lymph node assay: differentiating allergic and irritant responses using flow cytometry.
Local lymph node assay: differentiating allergic and irritant responses using flow cytometry.
复制标题
局部淋巴结测定：使用流式细胞术区分过敏和刺激反应。
作者：
G. Gerberick;L. Cruse;C. Ryan
鼠局部淋巴结评估（LLNA）是一种基于在接触敏感性响应的诱导阶段发生的事件来评估化学物质的接触敏感性的方法（DLN）局部暴露于化学物质后，就重要的皮肤传感器而言，LLNA至少与当前的豚鼠测试一样敏感，并且更可靠。在某些刺激物治疗后，我们观察到过敏剂与刺激性诱导的增殖，流式细胞仪技术已被用于检查DLN中的淋巴细胞亚群的表型在最终治疗后72小时收获DLN连续3天在耳朵上进行处理。在与过敏原细胞和刺激性小鼠中观察到了与幼稚和活化/记忆T细胞相关的两个粘合剂分子的细胞表达，CD62L（L-选择蛋白）和CD44（H-CAM）都会增加。相对于用过敏原处理的幼稚和媒介物处理的动物，与刺激性治疗的小鼠相比，B220（+）B细胞的百分比优先增加。表达T细胞激活/记忆表型CD62L（LO）CD44（HI）的CD4（+）和CD8（+）细胞的流式细胞仪，可以提供T细胞表型的流式细胞仪分析和T-cell活化标记的表达用于区分过敏原和刺激性诱导的化学处理小鼠DLN中的增殖反应的重要信息。
The murine local lymph node assay (LLNA) is a method for assessing the contact sensitization potential of chemicals. Based on events that occur during the induction phase of a contact sensitization response, the LLNA measures the in vivo proliferation of cells in the draining lymph nodes (DLNs) of mice following topical exposure to chemicals. In terms of predictive identification of important skin sensitizers, the LLNA has been shown to be at least as sensitive as, and much more reliable than, current guinea pig tests. However, proliferation has also been observed following treatment with some irritants. In an attempt to distinguish allergic from irritant-induced proliferation, flow cytometric techniques have been used to examine the phenotype of lymphocyte subsets in the DLNs as well as markers of T-lymphocyte activation and memory. Mice were treated on the ears for 3 consecutive days with allergens or irritants. The DLNs were harvested 72 h after the final treatment. Single-cell suspensions were prepared, counted, and stained for analysis of the percentages of T cells and B cells and T-cell expression of two adhesion molecules that have been associated with differentiating naïve and activated/memory T cells, CD62L (L-selectin) and CD44 (H-cam). Increases in lymph node cellularity were observed in both allergen- and irritant-treated mice relative to naïve and vehicle-treated animals. Mice treated with allergens showed a preferential increase in the percentage of B220(+) B cells compared with irritant-treated mice. Treatment with allergens, but not irritants, resulted in a selective increase in the percentages of CD4(+) and CD8(+) cells expressing the T-cell activation/memory phenotype CD62L(lo)CD44(hi). Taken together, flow cytometric analysis of cell phenotype and expression of T-cell activation/memory markers may provide important information for differentiating allergen- and irritant-induced proliferative responses in the DLNs of chemically treated mice.
由单克隆抗体 CZ-1 定义的 CD45RB 相关表位是小鼠 CD4 T 细胞的激活和记忆标记。
影响因子：
4.3
作者：
Varga,SM;Welsh,RM
通讯作者：
Welsh,RM
通过体内单克隆抗体耗竭揭示 CD4 和 CD8 T 细胞在小鼠接触敏感性中的作用。
DOI：
--
发表时间：
1990
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Gocinski,BL;Tigelaar,RE
通讯作者：
Tigelaar,RE