Use of a cloned library for the study of abundant poly(A)+RNA during Xenopus laevis development.
Use of a cloned library for the study of abundant poly(A)+RNA during Xenopus laevis development.
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使用克隆文库研究非洲爪蟾发育过程中丰富的多聚 (A) RNA。
DOI：
10.1016/0012-1606(80)90393-0
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发表时间：
1980
影响因子：
2.7
通讯作者：
Igor B. Dawid
中科院分区：
文献类型：
--
作者：
M. Dworkin;Igor B. Dawid
通过与从几个发育阶段的聚(A)+RNA制备的[32P]cDNA进行菌落杂交，分析了从非洲爪蟾胚胎聚(A)+RNA制备的重组DNA文库中的200多个克隆序列。早期胚胎发生期一直延伸到原肠胚形成开始（第 10 阶段），其特点是丰富的 Poly(A)+RNA 群体的相对恒定性。在原肠胚和尾芽阶段（第 24 阶段）之间，丰富的 Poly(A)+RNA 种类的模式发生了巨大变化；新的模式在蝌蚪前期摄食后期（第 41 阶段）的发育过程中至少 2 天保持相当稳定。我们还比较了两个不同阶段的非多聚体和多聚体 Poly(A)+RNA 群体。在第 10 阶段（原肠胚早期），核糖体后（游离核糖核蛋白）和多聚体 Poly(A)+RNA 群体部分重叠；然而，许多克隆序列在一个部分或另一个部分中以完全不同的浓度出现。在原肠胚主要为非多核体的序列中，很少有在尾芽阶段变成主要为多核体的序列。因此，我们没有证据表明随着发育的进展，丰富的非多聚核糖核酸大量招募到多聚核糖体中。我们宁愿观察到一种普遍模式，其中在一个发育阶段是非多聚体的克隆序列在其他阶段也往往是非多聚体的（如果可以检测到的话）。
Over 200 cloned sequences from recombinant DNA libraries prepared fromXenopus laevisembryonic poly(A)+RNA have been analyzed by colony hybridization with [32P]cDNA prepared from poly(A)+RNA from several stages of development. The period of early embryogenesis extending through the beginning of gastrulation (stage 10) is marked by the relative constancy of the abundant poly(A)+RNA population. Between the gastrula and tailbud stages (stage 24) there is a dramatic change in the pattern of abundant poly(A)+RNA species; the new pattern remains fairly constant for at least 2 days of development to the late prefeeding tadpole stages (stage 41). We have also compared nonpolysomal and polysomal poly(A)+RNA populations at two different stages. In stage 10 (early gastrula) postribosomal (free ribonucleoprotein) and polysomal poly(A)+RNA populations partly overlap; however, many cloned sequences occur in quite different concentrations in one fraction or the other. Among the sequences that are predominantly nonpolysomal at gastrula few become predominantly polysomal at tailbud stages. Thus, we have no evidence for a major recruitment of abundant nonpolysomal RNAs into polysomes with progressing development. We rather observe a general pattern in which a cloned sequence that is nonpolysomal in one stage of development tends to be nonpolysomal (if detectable at all) in other stages as well.