Off-line Arc transcription in active ensembles during fear memory retrieval.
Off-line Arc transcription in active ensembles during fear memory retrieval.
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恐惧记忆检索期间活跃整体中的离线 Arc 转录。
DOI：
10.1111/j.1460-9568.2012.08269.x
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Yamasaki Y
中科院分区：
文献类型：
--
作者：
Minoru Honda*;Hidetoshi Urakubo*;Takuya koumura;Shinya Kuroda;Miura Y;浦久保秀俊;Yamasaki Y
杏仁核记忆恢复后休息期间的神经活动和新生蛋白合成对于稳定重新激活的恐惧记忆是必要的。Arc/Arg3.1(Arc) 表达受神经活动调节，是记忆重新巩固的关键蛋白。然而，目前尚不清楚记忆检索是否会在随后的休息期间改变 Arc 转录。在本研究中，使用荧光原位杂交 (ArccatFISH) 时间活动的弧细胞区室分析 (ArccatFISH) 检测在记忆检索和休息时转录弧细胞的小鼠外侧杏仁核神经元群体。结果表明，记忆提取会改变神经元群的组成，从而在随后的休息期间激活 Arc 转录。大约 50% 转录 Arccat 的神经元在随后的休息期间在记忆检索过程中转录 Arcd，而只有大约 10% 在记忆检索之前的休息期间转录 Arcd 的神经元在记忆检索过程中转录 Arcd。相比之下，在受到电击但回忆起较少条件性恐惧的潜在抑制小鼠中，重新暴露于该室中会导致较少的优先Arc转录。总而言之，这些发现表明，在恐惧记忆检索过程中激活的神经元亚群优先在随后的外侧杏仁核休息期间进行转录。这种优先的Arc转录可能有助于记忆重新巩固。
Neural activity andde novoprotein synthesis during a rest period following memory retrieval in the amygdala is necessary for stabilization of reactivated fear memory.Arc/Arg3.1(Arc) expression is regulated by neural activity and is a critical protein for memory reconsolidation. However, it remains unclear whether memory retrieval altersArctranscription during subsequent rest. In this study, the populations of mouse lateral amygdala neurons that transcribeArcduring memory retrieval and at rest were detected usingArccellular compartment analysis of temporal activity by fluorescencein situhybridization (ArccatFISH). Results demonstrated that memory retrieval alters the composition of neuronal populations, which activateArctranscription during subsequent rest. Approximately 50% of neurons that transcribeArcat subsequent rest, transcribedArcduring memory retrieval, whereas only approximately 10% of neurons that transcribedArcduring a rest period prior to memory retrieval transcribeArcduring memory retrieval. In contrast, re‐exposure to the chamber induced less preferentialArctranscription in latent inhibited mice that received shocks but recalled less conditioned fear. Taken together, these findings indicate that neuronal subpopulations activated during fear memory retrieval preferentially transcribeArcduring subsequent rest in the lateral amygdala. This preferentialArctranscription may contribute to memory reconsolidation.