Downregulation of the expression of inhibin α subunit and betaglycan in porcine cystic follicles.
Downregulation of the expression of inhibin α subunit and betaglycan in porcine cystic follicles.
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DOI：
10.1292/jvms.14-0617
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发表时间：
2015-11
期刊：
影响因子：
--
通讯作者：
Zhou X
中科院分区：
文献类型：
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作者：
Wang C;Li C;Li H;Ma W;Chen S;Zhao Y;Rao J;Zhou X
抑制素作为转化生长因子-β超家族的成员，以内分泌的方式下调卵泡刺激素的合成和分泌。抑制素/β-聚糖在卵巢调节中的作用最近引起了人们的关注。到目前为止，还没有关于抑制素α亚单位和β-聚糖在囊性卵泡中的作用的数据。本研究采用免疫组织化学、实时荧光定量α和免疫印迹分析方法，研究了囊性卵泡中抑制素亚单位和β-葡聚糖的表达。抑制素α亚单位和β-葡聚糖免疫反应主要定位于卵泡颗粒细胞。与正常大卵泡相比，囊性卵泡中抑制素α亚单位和β-葡聚糖的表达较弱。而酶联免疫吸附试验结果显示，囊性卵泡液中抑制素α亚单位的浓度与对照组相比无显著差异(P>；0.05)。本研究探讨了卵泡刺激素(FSH)对体外培养的颗粒细胞表达β-多聚糖的影响。不出所料，在外源性FSH(30 ng/ml)作用下，颗粒细胞中β-葡聚糖基因和蛋白的表达显著增加(P<；0.05)。因此，本研究提供了抑制素α亚单位和β-聚糖在囊性卵泡中表达较弱的证据，这可能是由于囊性卵泡的存在导致卵泡分泌减少所致。
Inhibins, as members of the transforming growth factor beta (TGF-β) superfamily, downregulate the synthesis and secretion of follicle-stimulating hormone (FSH) in an endocrine manner. The role of inhibin/betaglycan in the ovary regulation recently gained attention. To date, no data exist on the function of inhibin α subunit and betaglycan in cystic follicles. In this study, the expressions of inhibin α subunit and betaglycan in cystic follicles were investigated using immunohistochemistry, real-time PCR and Western blot analysis. Both inhibin α subunit and betaglycan immunoreactivities were mainly localized in the granulosa cells of follicles. Expression of inhibin α subunit and betaglycan was inferior in cystic follicles compared with that in normal large follicles. However, the result of enzyme-linked immunosorbent assay showed no significant difference in the decreasing in concentration of inhibin α subunit in cystic follicular fluid compared with the control (P> 0.05). In this study, we explored the effects of FSH on betaglycan expression in granulosa cells in vitro. As expected, a significant increase in the expressions of betaglycan mRNA and protein in granulosa cells was observed in response to exogenous FSH (30 ng/ml)(P< 0.05) compared with the control. Consequently, this study provides evidence that the expressions of inhibin α subunit and betaglycan are inferior in cystic follicles, and this may be caused by the decrease in FSH in the presence of a cystic follicle.