Completely distinguishing individual a-genome chromosomes and their Karyotyping analysis by multiple bacterial artificial chromosome-fluorescence in situ hybridization
Completely distinguishing individual a-genome chromosomes and their Karyotyping analysis by multiple bacterial artificial chromosome-fluorescence in situ hybridization
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多细菌人工染色体-荧光原位杂交完全区分个体A基因组染色体及其核型分析
DOI：
10.1534/genetics.107.083576
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发表时间：
2008-02-01
期刊：
影响因子：
3.3
通讯作者：
Zhang, Tianzhen
中科院分区：
文献类型：
--
作者：
Wang, Kai;Guan, Bing;Zhang, Tianzhen
从亚洲棉（Gossypiumarboreumcv.）JLZM和G.多毛类植物TM-1和0-613- 2 R。结合45 S和5SrDNA，构建了一个22探针的混合物，可以识别所有13个G. arboreum染色体同时发育。根据它们与四倍体棉花的同源性，G. arboreum染色体命名为Al-Al 3，G. Arboreum介绍。这些结果表明，多重BAC-FISH在棉花细胞遗传学研究中的应用和技术，以克服在有丝分裂棉花细胞的染色体同时识别的问题。
Twenty bacterial artificial chromosome (BAC) clones that could produce bright signals and no or very low fluorescence in situ hybridization (FISH) background were identified from Gossypium arboreum cv. JLZM, and G. hirsutum accession (acc.) TM-1 and 0-613-2R. Combining with 45S and 5S rDNA, a 22-probe cocktail that could identify all 13 G. arboreum chromosomes simultaneously was developed. According to their homology with tetraploid cotton, the G. arboreum chromosomes were designated as Al-Al3, and a standard karyotype analysis of G. arboreum was presented. These results demonstrated an application for multiple BAC-FISH in cotton cytogenetic studies and a technique to overcome the problem of simultaneous chromosome recognition in mitotic cotton cells.