Nkx-2.5: a novel murine homeobox gene expressed in early heart progenitor cells and their myogenic descendants.
Nkx-2.5: a novel murine homeobox gene expressed in early heart progenitor cells and their myogenic descendants.
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DOI：
10.1242/dev.119.2.419
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发表时间：
1993-10
期刊：
影响因子：
4.6
通讯作者：
T. Lints;L. Parsons;L. Hartley;I. Lyons;R. Harvey
中科院分区：
文献类型：
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作者：
T. Lints;L. Parsons;L. Hartley;I. Lyons;R. Harvey
我们分离了两个小鼠同源框基因 Nkx-2.5 和 Nkx-2.6，它们是与果蝇 NK2、NK3 和 NK4/msh-2 相关的同源框基因 sp 亚家族的新成员。在本文中，我们重点关注 Nkx-2.5 基因及其在植入后发育过程中的表达模式。 Nkx-2.5 转录物首先在心肌祖细胞的头褶早期阶段被检测到。表达先于肌原性分化开始，并在胚胎、胎儿和成人心脏的心肌细胞中持续存在。在未来的咽内胚层中也检测到了转录本，该组织被认为可以产生心脏诱导剂。在内胚层中的表达仅在侧面发现，它与原心肌直接并列，表明两种组织之间存在相互作用。前肠闭合后，内胚层中的 Nkx-2.5 表达仅限于咽底，即发育中的心管的背面。甲状腺原基是咽底的衍生物，在咽部其余部分的转录水平减少后继续表达 Nkx-2.5。 Nkx-2.5 转录本也在舌肌、脾脏和胃中检测到。表达数据暗示Nkx-2.5参与心肌谱系的定向和/或分化。数据进一步表明，可以通过原肠胚形成晚期在分子水平上区分心源性祖细胞。因此，Nkx-2.5 表达将成为中胚层发育分析中的一个有价值的标记，也是剖析心脏肌发生分子基础的早期切入点。
We have isolated two murine homeobox genes, Nkx-2.5 and Nkx-2.6, that are new members of a sp sub-family of homeobox genes related to Drosophila NK2, NK3 and NK4/msh-2. In this paper, we focus on the Nkx-2.5 gene and its expression pattern during post-implantation development. Nkx-2.5 transcripts are first detected at early headfold stages in myocardiogenic progenitor cells. Expression preceeds the onset of myogenic differentiation, and continues in cardiomyocytes of embryonic, foetal and adult hearts. Transcripts are also detected in future pharyngeal endoderm, the tissue believed to produce the heart inducer. Expression in endoderm is only found laterally, where it is in direct apposition to promyocardium, suggesting an interaction between the two tissues. After foregut closure, Nkx-2.5 expression in endoderm is limited to the pharyngeal floor, dorsal to the developing heart tube. The thyroid primordium, a derivative of the pharyngeal floor, continues to express Nkx-2.5 after transcript levels diminish in the rest of the pharynx. Nkx-2.5 transcripts are also detected in lingual muscle, spleen and stomach. The expression data implicate Nkx-2.5 in commitment to and/or differentiation of the myocardial lineage. The data further demonstrate that cardiogenic progenitors can be distinguished at a molecular level by late gastrulation. Nkx-2.5 expression will therefore be a valuable marker in the analysis of mesoderm development and an early entry point for dissection of the molecular basis of myogenesis in the heart.