No Evidence for Cardiomyocyte Number Expansion in Preadolescent Mice
No Evidence for Cardiomyocyte Number Expansion in Preadolescent Mice
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DOI：
10.1016/j.cell.2015.10.035
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发表时间：
2015-11-05
期刊：
影响因子：
64.5
通讯作者：
Bergmann, Olaf
中科院分区：
文献类型：
--
作者：
Alkass, Kanar;Panula, Joni;Bergmann, Olaf
成人心脏中的心肌细胞生成的数量一直备受争议。最近的一份报告表明，在小鼠青春期前，心肌细胞的增殖导致心肌细胞数量增加40%。这样的扩张将改变我们对心脏生长的理解，并对心脏再生产生深远的影响。在这里，使用基于设计的体视学，我们发现心肌细胞增殖占出生后DNA合成的30%；然而，我们无法检测到出生后第11天后心肌细胞数量的任何变化。N-15-胸腺嘧啶核苷和BrdU分析没有提供青春期前小鼠出现增殖高峰的证据。相比之下，心肌细胞多核化占出生后DNA合成的57%，其次是心肌细胞核多倍化，在出生后第二和第三周内对DNA合成的贡献率为13%。我们得出的结论是，大多数心肌细胞在出生后第一周内形成，这一事件之后是两波非复制性DNA合成。本文是对Naqvi等人的回应。(2014)，发表于《细胞》杂志。另见Soonpaa等人的相关函件。(2015)，以及Naqvi等人的答复。(2015)，本期刊发。
The magnitude of cardiomyocyte generation in the adult heart has been heavily debated. A recent report suggests that duringmouse preadolescence, cardiomyocyte proliferation leads to a 40% increase in the number of cardiomyocytes. Such an expansion would change our understanding of heart growth and have far-reaching implications for cardiac regeneration. Here, using design-based stereology, we found that cardiomyocyte proliferation accounted for 30% of postnatal DNA synthesis; however, we were unable to detect any changes in cardiomyocyte number after postnatal day 11. N-15-thymidine and BrdU analyses provided no evidence for a proliferative peak in preadolescent mice. By contrast, cardiomyocyte multinucleation comprises 57% of postnatal DNA synthesis, followed by cardiomyocyte nuclear polyploidisation, contributing with 13% to DNA synthesis within the second and third postnatal weeks. We conclude that the majority of cardiomyocytes is set within the first postnatal week and that this event is followed by two waves of non-replicative DNA synthesis. This Matters Arising paper is in response to Naqvi et al. (2014), published in Cell. See also the associated Correspondence by Soonpaa et al. (2015), and the response by Naqvi et al. (2015), published in this issue.