Retention of functional chloroplasts in some sacoglossans from the Indo-Pacific and Mediterranean
Retention of functional chloroplasts in some sacoglossans from the Indo-Pacific and Mediterranean
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DOI：
10.1007/s00227-007-0648-6
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发表时间：
2007-07-01
期刊：
影响因子：
2.4
通讯作者：
Waegele, Heike
中科院分区：
文献类型：
--
作者：
Evertsen, Jussi;Burghardt, Ingo;Waegele, Heike
以往的研究kleptoplasty在sacoglossans使用不同的方法来调查多久sacoglossans能够保持光合活性（功能）叶绿体。在这项研究中，我们已经使用脉冲幅度调制荧光法来测量光系统II中的电荷分离的量子产率在黑暗驯化的细胞（Phi（IIe）），以检测光合活性的状态。本文报道了囊舌藻、眼斑板鳃藻、短突Elysia timida、Elysia sp、绒毛Elysia tomentosa、卡氏瑟氏线虫Thuridilla carlsoni、卡氏瑟氏线虫T.对Lineolata和Elysiella pusilla的功能性叶绿体（RFC）的保留能力进行了研究。结果表明，在三个不同的RFC的水平，其中P. ocellatus的RFC最长的超过11个月，E。Timida的RFC比P. ocellatus的1/4（几乎3个月），其余的RFC低至P. ocellatus的1/22（长达15天）。基于这些结果，并与以前的研究相比，八个不同水平的保留能力的非功能性和功能性叶绿体的sacoglossans提出。据我们所知，这是一个新的方法研究叶绿体功能的糖。
Previous studies on kleptoplasty in sacoglossans have used different methodology to investigate how long the sacoglossans are able to keep photosynthetically active (functional) chloroplasts. In this study we have used Pulse Amplitude Modulated Fluorometry to measure the quantum yield of charge separation in photosystem II in dark acclimated cells (Phi(IIe)) to detect the status of photosynthetic activity. Seven species of sacoglossa, Plakobranchus ocellatus, Elysia timida, Elysia sp, Elysia tomentosa, Thuridilla carlsoni, T. lineolata and Elysiella pusilla, were investigated regarding their ability to retain functional chloroplasts (RFC). The results show three different levels of RFC's where P. ocellatus has the longest RFC for more than 11 months, E. timida with a RFC 1/4 than P. ocellatus (almost 3 months) and the rest with RFC's down to 1/22 of P. ocellatus (up to 15 days). Based on these results, and compared to previous studies, eight different levels of retention abilities of non-functional and functional chloroplasts in sacoglossans are proposed. As far as we know, this is a novel method studying chloroplast functionality in sacoglossans.