Proteome of melamine urinary bladder stones and implication for stone formation
Proteome of melamine urinary bladder stones and implication for stone formation
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DOI：
10.1016/j.toxlet.2012.05.017
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发表时间：
2012-08-03
影响因子：
3.5
通讯作者：
Zhuang, Zhi-xiong
中科院分区：
文献类型：
--
作者：
Liu, Jian-dong;Liu, Jian-jun;Zhuang, Zhi-xiong
三聚氰胺可引起与肾病、膀胱增生或膀胱癌有关的泌尿系结石，但结石形成的机制尚不清楚。采用三聚氰胺染毒雄性大鼠13周，建立三聚氰胺膀胱结石模型，采用傅里叶变换红外光谱（FTIR）、X射线粉末衍射（XRD）、能量色散X射线（EDX）、十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）、液相色谱/质谱/质谱（LC-MS/MS）和western blot等方法对结石进行分析。分别从结石的组成和蛋白质组两个方面探讨蛋白质在结石形成中的作用。结果表明，膀胱结石主要由三聚氰胺和少量蛋白质组成。这些蛋白质的分子量范围很广，共鉴定出1051种蛋白质。基因本体论（GO）分类表明，大多数蛋白质来自受损细胞，参与各种代谢过程，并具有结合功能。在鉴定的蛋白质中，有一些炎症蛋白和尿蛋白。蛋白质的理化性质表明，67.1%的蛋白质等电点（pI）值在7.0以下，91.1%的蛋白质总平均疏水性（GRAVY）值在0以下，近一半的蛋白质是稳定的。我们的数据表明蛋白质可能在三聚氰胺膀胱结石的形成中起重要作用。(C)2012爱思唯尔爱尔兰有限公司保留所有权利。
Melamine can cause urinary stones related to nephropathy of the kidney and hyperplasia or carcinoma of the bladder, but the mechanism of stone formation is not well understood. In this study, male rats were administered melamine for thirteen weeks to establish melamine bladder stone models and the stones were analysed by Fourier transform infrared (FTIR) spectroscopy, powder X-ray diffraction (XRD), energy dispersive X-ray (EDX) spectroscopy, sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), liquid chromatography/mass spectrometry/mass spectrometry (LC-MS/MS) and western blot. respectively, for the composition and proteome, and to explore the implication of proteins for stone formation. The results showed bladder stones were composed of predominant melamine and a few amount of proteins. The proteins had a wide range of molecular weights and 1051 proteins were identified. Gene Ontology (GO) classification of the identified proteins showed most proteins were from injured cells, involved in various metabolic processes and had binding functions. Of the identified proteins, there were a few inflammatory proteins and urinary proteins. Physicochemical characteristics of the identified proteins showed that 67.1% proteins' isoelectric points (pI) value was below 7.0, 91.1% proteins' grand average of hydropathicity (GRAVY) scores were below 0 and nearly half of the proteins were stable. Our data indicated proteins might play an important role in melamine bladder stone formation. (C) 2012 Elsevier Ireland Ltd. All rights reserved.