Respiratory syncytial virus recombinant F protein (residues 255-278) induces a helper T cell type 1 immune response in mice.
Respiratory syncytial virus recombinant F protein (residues 255-278) induces a helper T cell type 1 immune response in mice.
复制标题
呼吸道合胞病毒重组 F 蛋白（残基 255-278）在小鼠中诱导辅助 T 细胞 1 型免疫反应。
DOI：
10.1089/vim.2007.0008
复制
发表时间：
2007
期刊：
影响因子：
2.2
通讯作者：
Moore,EddieG
中科院分区：
文献类型：
--
作者：
Singh,ShreeR;Dennis,VidaA;Carter,ChristinaL;Pillai,ShreekumarR;Moore,EddieG
We have developed and evaluated an immunodominant respiratory syncytial virus (RSV) F antigen in a mouse model. The antigenic region corresponding to amino acids 255–278 of the RSV F protein was cloned into a vector containing thectxA2Bgene of cholera toxin (CT). The recombinant protein was expressed inEscherichia coliand analyzed on sodium dodecyl sulfate–polyacrylamide gels. The purified protein was evaluated by immunoblot and ganglioside GM1enzyme-linked immunosorbent assay to confirm the expression of the RSV F protein and to correct association of the recombinant protein to form a holotoxin-like chimera, respectively. We hypothesized that genetic fusion of modified CT-based adjuvant with RSV F immunodominant epitopes (rRF-255) would induce protective humoral and cellular immune responses in mice. Intranasal immunization of mice with rRF-255 overall induced higher concentrations of anti-RSV F-specific antibodies in both serum and saliva as compared with mice immunized intranasally with RSV or phosphate-buffered saline (PBS). Antibody isotype analysis (IgA, IgG1, IgG2a, and IgG2b) was also performed. The predominant IgG2a antibody isotype response in combination with cytokine analysis of helper T cell type 1 (interferon-γ, interleukin [IL]-2, IL-12 p70, and tumor necrosis factor-α) and helper T cell type 2 (IL-4 and IL-10) responses revealed that rRF-255 antigen induces a prominent helper T cell type 1 immune response in mice. The rRF-255 antigen also induced serum neutralizing antibodies in immunized mice. Analysis of RSV load in lungs showed that rRF-255 immunization provided significant protection compared with PBS control animals.
登录
查看更多内容
影响因子：
2.9
作者：
EPAND, RM;BOTTEGA, R
通讯作者：
BOTTEGA, R
磷脂酰胆碱和胆固醇的二元混合物中的相平衡。
影响因子：
2.9
作者：
D. Recktenwald;H. Mcconnell
通讯作者：
H. Mcconnell
影响因子：
2.9
作者：
DUZGUNES, N;STRAUBINGER, RM;PAPAHADJOPOULOS, D
通讯作者：
PAPAHADJOPOULOS, D
通过抗原结合使靶标敏感的免疫脂质体不稳定——病毒的快速检测。
DOI：
10.1016/s0006-291x(86)80585-x
发表时间：
1986
影响因子：
3.1
作者：
Ho,RJ;Rouse,BT;Huang,L
通讯作者：
Huang,L
DOI：
10.1016/s0021-9258(17)44020-8
发表时间：
1983-11
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
A. Huang;S. Kennel;L. Huang
通讯作者：
A. Huang;S. Kennel;L. Huang