Daunomycin inverts the long-range chirality of DNA condensed states.
Daunomycin inverts the long-range chirality of DNA condensed states.
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道诺霉素可反转 DNA 凝聚态的长程手性。
DOI：
10.1021/bi00237a004
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发表时间：
1991
期刊：
影响因子：
2.9
通讯作者：
Builes,E
中科院分区：
文献类型：
--
作者：
Bustamante,C;Samorì,B;Builes,E
化学系，新墨西哥州，阿尔伯克基，新墨西哥州87131，和Dipartimento di Chimica Organica，博洛尼亚大学，viale Risorgimento 4，40136博洛尼亚，意大利1990年12月3日收到摘要：用圆二色谱（CD）和圆强度微分散射（CIDS）研究了柔红霉素对聚乙二醇（PEG）诱导的DNA凝聚态的影响。这些聚集体的CD光谱显示出psi型异常，强度比不存在PEG的分散DNA溶液获得的强度高10-100倍。增加浓度的柔红霉素，加入到DNA溶液之前，其聚集，导致，在PEG的存在下，CD和CIDS信号的幅度逐渐下降，并最终反转的迹象。两个信号的转变点的重合和在转变点处的CD光谱的仔细表征清楚地表明，观察到的反转对应于聚集体的手性的反转。后者的结果表明，在反转点的聚集体的结构应该类似于一个非线性液晶结构。在这种情况下获得的特征B-DNA光谱进一步表明，包装过程并不影响DNA分子的结构，其局部结构的微小变化可以引起其长程三级包装的显着变化。在这项研究中获得的结果代表了一个最近的理论的PSI型CD的异常信号被解释为一种表现的长程手性的聚集体的确认。
Department of Chemistry, University of New Mexico, Albuquerque, New Mexico 87131, and Dipartimento di Chimica Organica, University of Bologna, viale Risorgimento 4, 40136 Bologna, Italy Received December 3, 1990 abstract: The effect of daunomycin upon DNA condensed states induced bypoly (ethylene glycol)(PEG) was studied by circular dichroism (CD) and circular intensity differential scattering (CIDS). The CD spectra of these aggregates showedpsi-type anomalies and intensities 10-100 times greater than those obtained with the dispersed DNA solutions in the absence of PEG. Increasing concentrations of daunomycin, added to the DNA solution prior to its aggregation, led, in the presence of PEG, to CD and CIDS signals which gradually decreased in magnitude and eventually inverted sign. The coincidence of the transition point of both signals and a careful characterization of the CD spectrum at the transition point clearly indicated that the inversion observed corresponds to an inversion of the handedness of the aggregates. The latter result suggests that the structure of the aggregates at the inversion point should resemble that of a nematic liquid-crystalline structure. The characteristic B-DNA spectrum obtained in this case further suggests that the packing process does not affect thesecondary structure of the DNA molecules and that small changes in their local structure can induce dramatic changes in their long-range tertiary packing. The results obtained in this study represent a confirmation of a recent theory of psi-type CD in which the anomalous signals are interpreted as a manifestation of the long-range chirality of the aggregates.
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