Characterization of the fecal microbiota differs between age groups in Koreans
Characterization of the fecal microbiota differs between age groups in Koreans
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DOI：
10.5217/ir.2018.16.2.246
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发表时间：
2018-04-01
影响因子：
4.9
通讯作者：
Kim, Seokjin
中科院分区：
文献类型：
--
作者：
Kook, Sun-Young;Kim, Yunjeong;Kim, Seokjin
背景/目的：数万亿的微生物构成了人体的肠道微生物群。微生物群在维持宿主免疫和代谢方面起着关键作用。在韩国，肠道微生物组成的分析仅限于少数研究，包括小样本量。为了研究健康韩国人大样本的肠道微生物群落，我们分析了4种代表性细菌属乳杆菌属、双歧杆菌属、拟杆菌属和梭菌属的16 S核糖体RNA。方法：采用实时荧光定量聚合酶链反应技术对164例婴幼儿和214例成人的378份DNA样本进行分析。结果如下：对乳杆菌属、双歧杆菌属、拟杆菌属和梭菌属4种代表性细菌的16 S核糖体RNA分析表明，婴儿肠道菌群中双歧杆菌属和乳杆菌属的相对丰度高于成人，且以拟杆菌属和梭菌属为主。结论：据我们所知，这是第一项评估韩国婴儿和成人微生物群落独特特征的研究。这两个群体之间的差异表明，外部因素，如年龄，饮食和环境是重要的影响因素，在发展过程中肠道微生物组成的变化。
Background/Aims: Tens of trillions of microorganisms constitute the gut microbiota of the human body. The microbiota plays a critical role in maintaining host immunity and metabolism. Analyses of the gut microbial composition in Korea are limited to a few studies consisting of small sample sizes. To investigate the gut microbial community in a large sample of healthy Koreans, we analyzed the 16S ribosomal RNA of 4 representative bacterial genera Lactobacillus, Bifidobacterium, Bacteroides, and Clostridium. Methods: A total of 378 DNA samples extracted from 164 infants and 214 adults were analyzed using quantitative real-time polymerase chain reaction. Results: Analysis of 16S ribosomal RNA of 4 representative bacterial genera Lactobacillus, Bifidobacterium, Bacteroides, and Clostridium showed that the gut microbiota in infants had higher relative abundances of Bifidobacterium and Lactobacillus than that in adults, which was dominated by Bacteroides and Clostridium. Conclusions: To the best of our knowledge, this was the first study evaluating the distinct characteristics of the microbial community of Korean infants and adults. The differences between the 2 populations suggest that external factors such as age, diet, and the environment are important contributing factors to the change in gut microbial composition during development.