The effect of pH on the structural evolution of accelerated biomimetic apatite
The effect of pH on the structural evolution of accelerated biomimetic apatite
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DOI：
10.1016/j.biomaterials.2003.12.037
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发表时间：
2004-10-01
期刊：
影响因子：
14
通讯作者：
Wu, BM
中科院分区：
文献类型：
--
作者：
Chou, YF;Chiou, WA;Wu, BM
经典的仿生磷灰石涂层工艺可以通过首先将基底浸入浓缩的模拟体液中来加速。5 x SBF（SBFI），37 ℃。以形成前体磷灰石球的初始涂层，随后转移到不含晶体生长抑制剂的第二5 × SBF（SBF 2）溶液中，以促进SBF 1衍生的前体磷灰石球向最终结晶磷灰石板的相变。由于SBF 1控制初始前体球的形成动力学和组成。我们假设SBFI溶液的pH也将影响SBF 2衍生的结晶磷灰石的最终结构。为了检验这一假设，将聚苯乙烯基底浸入具有不同pH（5.8或6.5）的SBF 1中，然后浸入相同的SBF 2（pH 6.0）中。所得磷灰石在羟基、磷酸盐和碳酸盐基团方面表现出类似的XRD峰、FTIR光谱;以及Ca/P原子比（对于SBF 1（5，8）磷灰石为1.42;对于SBF 1（6.5）磷灰石为1.48）。SEM、TEM和电子衍射表明，SBF 1（6.5）（pH 6.5）前驱体球转变为较大的单晶片，而SBF 1 5 8（pH 5.8）前驱体球在主要为球形的前驱体基底上形成微小的多晶线片状结构。(C)2004爱思唯尔有限公司保留所有权利。
The classic biomimetric apatite coating process can be accelerated by first immersing substrates into concentrated simulated body fluid. 5 x SBF (SBFI), at 37degreesC. to form an initial coating of precursor apatite spheres, and subsequently transferring to a second 5 x SBF (SBF2) solution which is devoid of crystal growth inhibitors to promote phase transformation of SBF1-derived precursor apatite spheres into final crystalline apatite plates. Since SBF1 governs the formation kinetics and composition of the initial precursor spheres. we hypothesized that the pH of the SBFI Solution will also influence the final structure of the SBF2-derived crystalline apatite. To test this hypothesis, polystyrene substrates were immersed into SBF1 with different pH (5.8 or 6.5), and then immersed into the identical SBF2 (pH 6.0). The resultant apatites exhibited similar 20 XRD peaks-, FTIR spectra in terms of hydroxyl, phosphate and carbonate groups; and Ca/P atomic ratio (1.42 for SBF1(5,8) apatite; 1.48 for SBF1(6.5) apatite). SEM, TEM and electron diffraction show that while SBF1(6.5) (pH 6.5) precursor spheres transform into larger, single crystals plates, SBF 1 5 8 (pH 5.8) precursor spheres developed minute, polycrystal line plate-like structures over predominantly spherical precursor substrate. (C) 2004 Elsevier Ltd. All rights reserved.