Localization of minoxidil sulfotransferase in rat liver and the outer root sheath of anagen pelage and vibrissa follicles.
Localization of minoxidil sulfotransferase in rat liver and the outer root sheath of anagen pelage and vibrissa follicles.
复制标题
米诺地尔磺基转移酶在大鼠肝脏以及毛发生长初期毛皮和触须毛囊的外根鞘中的定位。
DOI：
10.1111/1523-1747.ep12515856
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Diani,AR
中科院分区：
文献类型：
--
作者：
Dooley,TP;Walker,CJ;Hirshey,SJ;Falany,CN;Diani,AR
米诺地尔刺激头发生长的确切生化机制和作用部位尚不清楚。硫酸米诺地尔是米诺地尔的活性代谢物，对平滑肌血管扩张和毛发生长有促进作用。硫酸米诺地尔的形成是由特定的paps依赖性硫转移酶催化的，米诺地尔硫酸化活性先前报道存在于肝脏和毛囊中。从大鼠肝脏中纯化了其中一种米诺地尔硫酸化酶（鼠米诺地尔硫转移酶，MST），制备了兔抗MST抗体。利用这种抗mst抗体，我们在大鼠肝脏和毛囊中免疫组化定位了米诺地尔硫转移酶。在大鼠皮毛和毛囊中，这种酶定位于下外根鞘上皮细胞的细胞质内。虽然MST的免疫定位可能不一定与已知存在于毛囊中的MST活性相关，但本研究的结果强烈表明，毛囊的下外根鞘可能是局部应用米诺地尔磺化的一个部位。
The precise biochemical mechanism and site(s) of action by which minoxidil stimulates hair growth are not yet clear. Minoxidil sulfate is the active metabolite of minoxidil, with regard to smooth muscle vasodilation and hair growth. Formation of minoxidil sulfate is catalyzed by specific PAPS-dependent sulfotransferase(s) and minoxidil-sulfating activities have been previously reported to be present in liver and hair follicles. One of these minoxidil-sulfating enzymes has been purified from rat liver (rat minoxidil sulfotransferase, MST) and a rabbit anti-MST antibody has been prepared. Using this anti-MST antibody, we have immunohistochernically localized minoxidil sulfotransferase in the liver and anagen hair follicles from rat. In rat pelage and vibrissa follicles, this enzyme is localized within the cytoplasm of epithelial cells in the lower outer root sheath. Although the immunolocalization of MST might not necessarily correlate with the MST activity known to be present in anagen follicles, the results of this study strongly suggest that the lower outer root sheath of the hair follicle may serve as a site for the sulfation of topically applied minoxidil.