Modular Subdivision of Mushroom Bodies by Kenyon Cells in the Silkmoth
Modular Subdivision of Mushroom Bodies by Kenyon Cells in the Silkmoth
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DOI：
10.1002/cne.21946
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发表时间：
2009-03-20
影响因子：
2.5
通讯作者：
Kanzaki, Ryohei
中科院分区：
文献类型：
--
作者：
Fukushima, Ryota;Kanzaki, Ryohei
在昆虫中，第一嗅觉中枢触角叶小球中的嗅觉信息通过投射神经元传递到外侧前脑和蘑菇体的花萼。在雄蚕（家蚕），在花萼的树枝状模式显着不同的投射神经元，性信息素的反应和一般的气味。然而，人们对蘑菇体的内在神经元的组织知之甚少，这种神经元被称为凯尼恩细胞（KC），它接收来自投射神经元的输入。我们调查了蚕蛾蘑菇体，并确定了四个平行的细分在叶和花梗免疫标记的抗体对蛋白激酶A的催化亚基在果蝇（DCO）和神经调节肽FMRFamide。为了进一步了解蘑菇体的详细组织，我们对单个KC进行了广泛的标记。我们确定了四种形态类型的轴突投影对应于在叶的细分，并发现，每种类型的KC有一个特征性的树突状形态在花萼。这些结果表明，在叶和树突状形态在花萼的KC的轴突投射之间的相关性，并表示不同的功能作用的细分。神经学比较杂志513：315-330，2009. (C)2009威利-利斯公司
In insects, olfactory information in the glomeruli of the antennal lobe, the first olfactory center, is transmitted to the lateral protocerebrum and the calyx of the mushroom body via projection neurons. In male silkmoths (Bombyx mori), arborization patterns in the calyx differ markedly between projection neurons that respond to sex pheromones and those that respond to general odors. However, little is known about the organization of the mushroom body's intrinsic neurons, called Kenyon cells (KCs), which receive the inputs from the projection neurons. We investigated the silkmoth mushroom body and identified four parallel subdivisions in the lobes and pedunculus by immunolabeling with antibodies against the catalytic subunit of protein kinase A in Drosophila melanogaster (DCO) and the neuromodulatory peptide FMRFamide. To further understand the detailed organization of the mushroom body, we performed extensive labeling of individual KCs. We identified four morphological types whose axonal projections corresponded to the subdivisions in the lobes, and found that each type of KC had a characteristic dendritic morphology in the calyx. These results show a correlation between the axonal projections of KCs in the lobes and dendritic morphology in the calyx, and indicate different functional roles for the subdivisions. J. Comp. Neurol. 513:315-330, 2009. (C) 2009 Wiley-Liss, Inc.