Fibronectin splice variation in human knee cartilage, meniscus and synovial membrane: observations in osteoarthritic knee.
Fibronectin splice variation in human knee cartilage, meniscus and synovial membrane: observations in osteoarthritic knee.
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DOI：
10.1002/jor.22787
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发表时间：
2015-04
期刊：
影响因子：
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通讯作者：
Zhang Y
中科院分区：
文献类型：
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作者：
Scanzello CR;Markova DZ;Chee A;Xiu Y;Adams SL;Anderson G;Zgonis M;Qin L;An HS;Zhang Y
纤维连接蛋白（FN）是一种广泛表达的分子，可参与影响软骨、半月板和滑膜（SM）的骨关节炎（OA）的发展。FN在关节组织中的选择性剪接异构体，而不是软骨，以前没有被广泛研究。本研究比较了不同程度的骨关节炎患者的FN剪接变异。从无症状供体和接受关节镜手术的患者中收集关节组织。使用可变剪接的额外结构域A（EDA）、额外结构域B（EDB）和可变（V）区侧翼的引物通过PCR扩增总RNA。EDB+、EDB−、EDA −和V+变体存在于所有关节组织中，而EDA+变体很少检测到。EDB+和EDV+变体的表达水平在软骨、滑膜和关节组织中相似。关节镜检查患者滑膜中V+ FN的表达随软骨退变程度而变化。先前在软骨中鉴定的两种V−亚型也存在于SM和半月板中。半月板和SM中的纤连蛋白剪接与软骨中的纤连蛋白剪接具有惊人的相似性。滑膜V+ FN的表达水平随软骨退变程度而变化。V+ FN应在更大的人群中作为疾病分期或进展的潜在生物标志物进行研究。
Fibronectin (FN) is a widely expressed molecule that can participate in development of osteoarthritis (OA) affecting cartilage, meniscus, and synovial membrane (SM). The alternatively spliced isoforms of FN in joint tissues other than cartilage have not been extensively studied previously. The present study compares FN splice variation in patients with varying degrees of osteoarthritic change. Joint tissues were collected from asymptomatic donors and patients undergoing arthroscopic procedures. Total RNA was amplified by PCR using primers flanking alternatively spliced Extra Domain A (EDA), Extra Domain B (EDB) and Variable (V) regions. EDB+, EDB− and EDA− and V+ variants were present in all joint tissues, while the EDA+ variant was rarely detected. Expression levels of EDB+ and EDV+ variants were similar in cartilage, synovium and meniscal tissues. Synovial expression of V+ FN in arthroscopy patients varied with degree of cartilage degeneration. Two V− isoforms, previously identified in cartilage, were also present in SM and meniscus. Fibronectin splicing in meniscus and SM bears striking resemblance to that of cartilage. Expression levels of synovial V+ FN varied with degree of cartilage degeneration. V+ FN should be investigated as a potential biomarker of disease stage or progression in larger populations.