Comparative analysis of anti-mouse SRY antibodies
Comparative analysis of anti-mouse SRY antibodies
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DOI：
10.1159/000108933
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发表时间：
2007-01-01
影响因子：
2.3
通讯作者：
Koopman, P.
中科院分区：
文献类型：
--
作者：
Bradford, S. T.;Wilhelm, D.;Koopman, P.
Y染色体基因SRY是哺乳动物雄性性分化的启动子，但SRY下游的分子和细胞机制尚不清楚。对这些问题的更深入理解依赖于在许多实验条件下内源性地可视化SRY蛋白的能力。在这里，我们比较了几种可用的抗体对小鼠SRY的特异性和有效性。两种抗体交叉反应与其他SOX蛋白的转染细胞的免疫荧光分析，这两个是无法检测到SRY的Western印迹。第三种抗体是既亲又特异的，并且能够检测小鼠生殖嵴中发育中的支持细胞中的内源性SRY。我们的研究结果强调，需要区分有用的和虚假的生化和免疫定位研究涉及小鼠SRY蛋白的试剂。版权所有（C）2007 S. Karger AG，巴塞尔。
The Y chromosome gene SRY is the initiator of male sexual differentiation in mammals, but the molecular and cellular mechanisms operating downstream of SRY remain undefined. A deeper understanding of these issues relies on the ability to visualize SRY protein endogenously under a number of experimental conditions. Here we compare the specificity and effectiveness of several available antibodies to mouse SRY. Two antibodies cross-reacted with other SOX proteins in immunofluorescence analyses of transfected cells, and one of these two was unable to detect SRY on Western blots. A third antibody was both avid and specific, and was able to detect endogenous SRY in developing Sertoli cells in mouse genital ridges. Our findings underline the need to distinguish between useful and spurious reagents for biochemical and immunolocalization studies involving mouse SRY protein. Copyright (C) 2007 S. Karger AG, Basel.